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PMID: 9736555 Published · ppublish English Journal Article

Detection of Tn917-like sequences within a Tn916-like conjugative transposon (Tn3872) in erythromycin-resistant isolates of Streptococcus pneumoniae.

Antimicrobial agents and chemotherapy ·Vol. 42 ·No. 9 ·1998-09-00 ·Pages 2312-8

McDougal LK, Tenover FC, Lee LN, Rasheed JK, Patterson JE, Jorgensen JH, LeBlanc DJ

Abstract

A series of macrolide-lincosamide-streptogramin B (MLS)-resistant pneumococcal isolates of a variety of serotypes was examined and was found to contain Tn917-like elements by DNA-DNA hybridization. Like Tn1545, Tn917 also encodes an ermAM gene but does not mediate resistance to other antimicrobial agents. Furthermore, nucleotide sequence analyses of the DNAs flanking three of the Tn917-like elements revealed that they were inserted into orf9 of a Tn916-like element in a composite transposon-like structure (Tn3872). Other MLS-resistant strains appeared to contain Tn1545-like elements that had suffered a deletion of sequences including the aphA-3 sequences responsible for kanamycin resistance. Thus, the MLS resistance phenotype in pneumococci appears to be mediated by the ermAM present on a much wider variety of genetic elements than was previously appreciated.

MeSH Terms
Anti-Bacterial Agents/pharmacology Base Sequence Conjugation, Genetic DNA Transposable Elements DNA, Bacterial/chemistry Drug Resistance, Microbial Erythromycin/pharmacology Molecular Sequence Data Nucleic Acid Hybridization Polymerase Chain Reaction Streptococcus pneumoniae/drug effects,genetics
Chemicals
Anti-Bacterial Agents DNA Transposable Elements DNA, Bacterial Erythromycin
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
McDougal L K
Hospital Infections Program, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
Tenover F C
Lee L N
Rasheed J K
Patterson J E
Jorgensen J H
LeBlanc D J
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.42.9.2312
Published
1998-09-00
Pages
2312-8
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC105825
Subset
IM
Databases
GENBANK
AF066796, AF066797
External Links
PubMed source DOI: 10.1128/AAC.42.9.2312 PMC Full Text View journal details
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