Home LiteratureArticle Details
PMID: 9722639 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Biochemical and genetic characterization of the dominant positive element driving transcription ofthe yeast TBP-encoding gene, SPT15.

Nucleic acids research ·Vol. 26 ·No. 18 ·1998-09-15 ·Pages 4186-95

Schroeder SC, Weil PA

Abstract

We previously demonstrated that a combination of both positive and negative cis -acting upstream elements control the transcription of the gene encoding TBP ( SPT15 ) in Saccharomyces cerevisiae . One of these elements found in that study, resident between 5' flanking sequences -147 and -128 , and termed PED (for positive element distal), was found to play an essential positive role in driving transcription of the gene encoding TBP. In this report, we map at nucleotide-level resolution, the critical residues which comprise PED, purify and sequence the protein that binds to it and determine that this PED binding factor is Abf1p, an abundant yeast protein previously broadly implicated in both gene regulation and DNA replication. In the case of the TBP-encoding gene, however, Abf1p works through the PED element which is a non-consensus binding site. Based upon the work of others, the PED-variant ABF1 site would be predicted to be a very poor binding site for this factor yet Abf1p binds PED and a consensus ABF1 site with comparable affinity. These results are discussed in light of the broader context of Abf1p-mediated gene regulation.

MeSH Terms
Base Sequence Cell Cycle Proteins/biosynthesis,genetics,isolation & purification Chromatography, Affinity DNA Probes DNA-Binding Proteins/genetics,isolation & purification,metabolism DNA-Directed RNA Polymerases/genetics,metabolism Fungal Proteins/genetics,metabolism Gene Expression Regulation, Fungal Kinetics Molecular Sequence Data Mutagenesis, Site-Directed Recombinant Fusion Proteins/biosynthesis Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins TATA Box TATA-Box Binding Protein/analogs & derivatives Transcription Factors/genetics,isolation & purification,metabolism Transcription, Genetic beta-Galactosidase/biosynthesis,genetics
Chemicals
ABF1 protein, S cerevisiae Cell Cycle Proteins DNA Probes DNA-Binding Proteins Fungal Proteins Recombinant Fusion Proteins SPT15 protein, S cerevisiae Saccharomyces cerevisiae Proteins TATA-Box Binding Protein Transcription Factors DNA-Directed RNA Polymerases beta-Galactosidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Schroeder S C
Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville,TN 37232-0615, USA.
Weil P A
References (37)
37 references, click to expand
  1. Two DNA-binding factors recognize specific sequences at silencers, upstream activating sequences, autonomously replicating sequences, and telomeres in Saccharomyces cerevisiae.
    Mol Cell Biol. 1988 Jan;8(1):210-25 PMID: 3275867
  2. TATA-binding protein is limiting for both TATA-containing and TATA-lacking RNA polymerase III promoters in Drosophila cells.
    Mol Cell Biol. 1996 Dec;16(12):6909-16 PMID: 8943346
  3. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.
    Genetics. 1989 May;122(1):19-27 PMID: 2659436
  4. Purification and characterization of OBF1: a Saccharomyces cerevisiae protein that binds to autonomously replicating sequences.
    Mol Cell Biol. 1989 Jul;9(7):2906-13 PMID: 2674673
  5. A yeast ARS-binding protein activates transcription synergistically in combination with other weak activating factors.
    Mol Cell Biol. 1990 Mar;10(3):887-97 PMID: 2406570
  6. The ABF1 factor is the transcriptional activator of the L2 ribosomal protein genes in Saccharomyces cerevisiae.
    Mol Cell Biol. 1990 May;10(5):2437-41 PMID: 2183035
  7. Analysis of the human TATA binding protein promoter and identification of an ets site critical for activity.
    Nucleic Acids Res. 1997 Jun 15;25(12):2485-94 PMID: 9171103
  8. Yeast general transcription factor GFI: sequence requirements for binding to DNA and evolutionary conservation.
    Nucleic Acids Res. 1990 May 11;18(9):2769-76 PMID: 2187179
  9. Isolation and characterization of the Drosophila gene encoding the TATA box binding protein, TFIID.
    Cell. 1990 Jun 29;61(7):1179-86 PMID: 2194666
  10. High-performance affinity chromatography of DNA.
    J Chromatogr. 1990 Jun 1;508(2):279-87 PMID: 2370282
  11. ABF1 binding sites in yeast RNA polymerase genes.
    J Biol Chem. 1990 Sep 5;265(25):15168-75 PMID: 2203770
  12. Multifunctional DNA-binding proteins mediate concerted transcription activation of yeast ribosomal protein genes.
    Biochim Biophys Acta. 1990 Aug 27;1050(1-3):351-5 PMID: 2207166
  13. Cloning of the Schizosaccharomyces pombe TFIID gene reveals a strong conservation of functional domains present in Saccharomyces cerevisiae TFIID.
    Genes Dev. 1990 Jul;4(7):1141-8 PMID: 2210373
  14. Tackling the protease problem in Saccharomyces cerevisiae.
    Methods Enzymol. 1991;194:428-53 PMID: 2005802
  15. Detection of an unusual distortion in A-tract DNA using KMnO4: effect of temperature and distamycin on the altered conformation.
    Nucleic Acids Res. 1991 Jun 25;19(12):3421-9 PMID: 2062656
  16. The conserved carboxy-terminal domain of Saccharomyces cerevisiae TFIID is sufficient to support normal cell growth.
    Mol Cell Biol. 1991 Oct;11(10):4809-21 PMID: 1922021
  17. TFIID can be rate limiting in vivo for TATA-containing, but not TATA-lacking, RNA polymerase II promoters.
    Genes Dev. 1992 Feb;6(2):304-15 PMID: 1737620
  18. The TATA-binding protein and associated factors are integral components of the RNA polymerase I transcription factor, SL1.
    Cell. 1992 Mar 6;68(5):965-76 PMID: 1547496
  19. The TATA-binding protein is required for transcription by all three nuclear RNA polymerases in yeast cells.
    Cell. 1992 May 15;69(4):685-96 PMID: 1586947
  20. Variants of the TATA-binding protein can distinguish subsets of RNA polymerase I, II, and III promoters.
    Cell. 1992 May 15;69(4):697-702 PMID: 1586948
  21. Isolation of genomic DNA encoding transcription factor TFIID from Acanthamoeba castellanii: characterization of the promoter.
    Nucleic Acids Res. 1992 Sep 25;20(18):4817-24 PMID: 1408796
  22. Genetic and biochemical analyses of yeast TATA-binding protein mutants.
    J Biol Chem. 1993 Mar 5;268(7):5005-13 PMID: 8444878
  23. TBP, a universal eukaryotic transcription factor?
    Genes Dev. 1993 Jul;7(7B):1291-308 PMID: 8330735
  24. Structure of a mammalian TBP (TATA-binding protein) gene: isolation of the mouse TBP genome.
    Nucleic Acids Res. 1993 Jun 11;21(11):2769 PMID: 8332475
  25. Transcription factor TFIIB sites important for interaction with promoter-bound TFIID.
    Science. 1993 Jul 23;261(5120):463-6 PMID: 8332911
  26. Immunopurification of yeast TATA-binding protein and associated factors. Presence of transcription factor IIIB transcriptional activity.
    J Biol Chem. 1993 Jul 25;268(21):15325-8 PMID: 8340360
  27. An upstream promoter element of the Acanthamoeba castellanii TBP gene binds a DNA sequence specific transcription activating protein, TPBF.
    Nucleic Acids Res. 1993 Sep 11;21(18):4321-9 PMID: 8414988
  28. Purification and characterization of TATA-binding protein promoter binding factor. A regulatory transcription factor of the tbp gene.
    J Biol Chem. 1994 Jul 15;269(28):18541-8 PMID: 8034602
  29. Identification of the cis-acting DNA sequence elements regulating the transcription of the Saccharomyces cerevisiae gene encoding TBP, the TATA box binding protein.
    J Biol Chem. 1994 Nov 11;269(45):28335-46 PMID: 7961772
  30. Transcriptional activators differ in their responses to overexpression of TATA-box-binding protein.
    Mol Cell Biol. 1995 Mar;15(3):1554-63 PMID: 7862148
  31. Structure of the genes encoding transcription factor IIB and TATA box-binding protein from Drosophila melanogaster.
    Gene. 1995 Feb 14;153(2):203-7 PMID: 7875589
  32. ABF1 Ser-720 is a predominant phosphorylation site for casein kinase II of Saccharomyces cerevisiae.
    J Biol Chem. 1995 Jul 7;270(27):16153-9 PMID: 7608180
  33. Genomic structure of the human TATA-box-binding protein (TBP).
    Gene. 1995 Aug 19;161(2):277-82 PMID: 7665093
  34. Binding sites for abundant nuclear factors modulate RNA polymerase I-dependent enhancer function in Saccharomyces cerevisiae.
    J Biol Chem. 1995 Dec 1;270(48):28723-32 PMID: 7499394
  35. Cloning, expression, and characterization of the TATA-binding protein (TBP) promoter binding factor, a transcription activator of the Acanthamoeba TBP gene.
    J Biol Chem. 1995 Dec 1;270(48):28839-47 PMID: 7499409
  36. Participation of the yeast activator Abf1 in meiosis-specific expression of the HOP1 gene.
    Mol Cell Biol. 1996 Jun;16(6):2777-86 PMID: 8649386
  37. 5-Fluoroorotic acid as a selective agent in yeast molecular genetics.
    Methods Enzymol. 1987;154:164-75 PMID: 3323810
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/26.18.4186
Published
1998-09-15
Pages
4186-95
Language
English
Region
England
NLM ID
0411011
PMCID
PMC147844
Subset
IM
Grants
NIGMS NIH HHS · GM52461 · United States
External Links
PubMed source DOI: 10.1093/nar/26.18.4186 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2