Home LiteratureArticle Details
PMID: 2406570 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A yeast ARS-binding protein activates transcription synergistically in combination with other weak activating factors.

Molecular and cellular biology ·Vol. 10 ·No. 3 ·1990-03-00 ·Pages 887-97

Buchman AR, Kornberg RD

Abstract

ABFI (ARS-binding protein I) is a yeast protein that binds specific DNA sequences associated with several autonomously replicating sequences (ARSs). ABFI also binds sequences located in promoter regions of some yeast genes, including DED1, an essential gene of unknown function that is transcribed constitutively at a high level. ABFI was purified by specific binding to the DED1 upstream activating sequence (UAS) and was found to recognize related sequences at several other promoters, at an ARS (ARS1), and at a transcriptional silencer (HMR E). All ABFI-binding sites, regardless of origin, provided weak UAS function in vivo when examined in test plasmids. UAS function was abolished by point mutations that reduced ABFI binding in vitro. Analysis of the DED1 promoter showed that two ABFI-binding sites combine synergistically with an adjacent T-rich sequence to form a strong constitutive activator. The DED1 T-rich element acted synergistically with all other ABFI-binding sites and with binding sites for other multifunctional yeast activators. An examination of the properties of sequences surrounding ARS1 left open the possibility that ABFI enhances the initiation of DNA replication at ARS1 by transcriptional activation.

MeSH Terms
Base Sequence DNA Replication DNA-Binding Proteins/isolation & purification,physiology Fungal Proteins/isolation & purification,metabolism Gene Expression Regulation, Fungal Molecular Sequence Data Regulatory Sequences, Nucleic Acid Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Transcription Factors/isolation & purification,physiology Transcription, Genetic
Chemicals
ABF1 protein, S cerevisiae DNA-Binding Proteins Fungal Proteins Saccharomyces cerevisiae Proteins Transcription Factors
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Buchman A R
Department of Cell Biology, Beckman Laboratories, Stanford University School of Medicine, California 94305.
Kornberg R D
References (59)
59 references, click to expand
  1. Nucleosome reconstitution on plasmid-inserted poly(dA) . poly(dT).
    EMBO J. 1982;1(2):173-9 PMID: 6325153
  2. Isolation of a Saccharomyces cerevisiae centromere DNA-binding protein, its human homolog, and its possible role as a transcription factor.
    Mol Cell Biol. 1987 Jan;7(1):403-9 PMID: 3550420
  3. Activation of yeast RNA polymerase II transcription by a thymidine-rich upstream element in vitro.
    Proc Natl Acad Sci U S A. 1989 Jan;86(2):486-90 PMID: 2643115
  4. Bent DNA at a yeast autonomously replicating sequence.
    Nature. 1986 Nov 6-12;324(6092):87-9 PMID: 3785376
  5. Purification and cloning of a DNA binding protein from yeast that binds to both silencer and activator elements.
    Cell. 1987 Dec 4;51(5):721-32 PMID: 3315231
  6. Deletion mutations affecting autonomously replicating sequence ARS1 of Saccharomyces cerevisiae.
    Mol Cell Biol. 1984 Nov;4(11):2455-66 PMID: 6392851
  7. An RNA polymerase II transcription factor binds to an upstream element in the adenovirus major late promoter.
    Cell. 1985 Dec;43(2 Pt 1):439-48 PMID: 4075400
  8. A general upstream binding factor for genes of the yeast translational apparatus.
    EMBO J. 1985 Dec 16;4(13A):3539-47 PMID: 3912170
  9. TUF, the yeast DNA-binding factor specific for UASrpg upstream activating sequences: identification of the protein and its DNA-binding domain.
    Proc Natl Acad Sci U S A. 1987 Jun;84(11):3648-52 PMID: 3295867
  10. Trans-acting protein factors and the regulation of eukaryotic transcription: lessons from studies on DNA tumor viruses.
    Genes Dev. 1988 Mar;2(3):267-81 PMID: 3288540
  11. Statistical positioning of nucleosomes by specific protein-binding to an upstream activating sequence in yeast.
    J Mol Biol. 1988 Nov 5;204(1):109-27 PMID: 3063825
  12. Specific protein binding to far upstream activating sequences in polymerase II promoters.
    Proc Natl Acad Sci U S A. 1985 Jan;82(1):43-7 PMID: 3881758
  13. Nuclear factor III, a novel sequence-specific DNA-binding protein from HeLa cells stimulating adenovirus DNA replication.
    Nature. 1986 Aug 14-20;322(6080):656-9 PMID: 3748145
  14. The SV40 enhancer is composed of multiple functional elements that can compensate for one another.
    Cell. 1986 May 9;45(3):461-70 PMID: 3009027
  15. Connections between transcriptional activators, silencers, and telomeres as revealed by functional analysis of a yeast DNA-binding protein.
    Mol Cell Biol. 1988 Dec;8(12):5086-99 PMID: 3072472
  16. A short 5' flanking region containing conserved sequences is required for silkworm alanine tRNA gene activity.
    Proc Natl Acad Sci U S A. 1983 Jun;80(11):3416-20 PMID: 16593326
  17. The localization of replication origins on ARS plasmids in S. cerevisiae.
    Cell. 1987 Nov 6;51(3):463-71 PMID: 2822257
  18. Interaction of a gene-specific transcription factor with the adenovirus major late promoter upstream of the TATA box region.
    Cell. 1985 Nov;43(1):165-75 PMID: 4075392
  19. Identification of two factors which bind to the upstream sequences of a number of nuclear genes coding for mitochondrial proteins and to genetic elements important for cell division in yeast.
    Nucleic Acids Res. 1988 Aug 11;16(15):7287-301 PMID: 3045755
  20. A region flanking the GAL7 gene and a binding site for GAL4 protein as upstream activating sequences in yeast.
    J Mol Biol. 1985 Dec 20;186(4):821-4 PMID: 3912516
  21. Transcriptional activation of initiation of replication from the E. coli chromosomal origin: an RNA-DNA hybrid near oriC.
    Cell. 1988 Oct 7;55(1):113-23 PMID: 2458841
  22. Promoters, activator proteins, and the mechanism of transcriptional initiation in yeast.
    Cell. 1987 May 8;49(3):295-7 PMID: 2882858
  23. Transcription interferes with elements important for chromosome maintenance in Saccharomyces cerevisiae.
    Mol Cell Biol. 1988 May;8(5):2184-94 PMID: 3290652
  24. Cooperativity and hierarchical levels of functional organization in the SV40 enhancer.
    Cell. 1988 Sep 23;54(7):943-53 PMID: 2843294
  25. Purification of a yeast protein that binds to origins of DNA replication and a transcriptional silencer.
    Proc Natl Acad Sci U S A. 1988 Apr;85(7):2120-4 PMID: 3281162
  26. A common octamer motif binding protein is involved in the transcription of U6 snRNA by RNA polymerase III and U2 snRNA by RNA polymerase II.
    Cell. 1987 Oct 9;51(1):71-9 PMID: 3652209
  27. Nucleotide sequence and transcriptional mapping of the yeast pet56-his3-ded1 gene region.
    Nucleic Acids Res. 1985 Dec 9;13(23):8587-601 PMID: 3001645
  28. Naturally occurring poly(dA-dT) sequences are upstream promoter elements for constitutive transcription in yeast.
    Proc Natl Acad Sci U S A. 1985 Dec;82(24):8419-23 PMID: 3909145
  29. Isolation and characterization of the SPT2 gene, a negative regulator of Ty-controlled yeast gene expression.
    Mol Cell Biol. 1985 Jul;5(7):1543-53 PMID: 2991744
  30. A cellular DNA-binding protein that activates eukaryotic transcription and DNA replication.
    Cell. 1987 Jan 16;48(1):79-89 PMID: 3024847
  31. Two DNA-binding factors recognize specific sequences at silencers, upstream activating sequences, autonomously replicating sequences, and telomeres in Saccharomyces cerevisiae.
    Mol Cell Biol. 1988 Jan;8(1):210-25 PMID: 3275867
  32. The SV40 enhancer contains two distinct levels of organization.
    Nature. 1988 May 5;333(6168):40-5 PMID: 2834649
  33. Centromeric DNA from Saccharomyces cerevisiae.
    J Mol Biol. 1982 Jun 25;158(2):157-90 PMID: 6750136
  34. Silver staining of proteins in polyacrylamide gels.
    Anal Biochem. 1981 Nov 15;118(1):197-203 PMID: 6175245
  35. Roles of two DNA-binding factors in replication, segregation and transcriptional repression mediated by a yeast silencer.
    EMBO J. 1988 Jul;7(7):2241-53 PMID: 3046937
  36. Cooperativity of the glucocorticoid receptor and the CACCC-box binding factor.
    Nature. 1988 Mar 3;332(6159):87-90 PMID: 2831456
  37. Sigma elements are position-specific for many different yeast tRNA genes.
    Nucleic Acids Res. 1988 Feb 25;16(4):1499-515 PMID: 3279393
  38. Constitutive transcription of yeast ribosomal protein gene TCM1 is promoted by uncommon cis- and trans-acting elements.
    Mol Cell Biol. 1988 Oct;8(10):4328-41 PMID: 3054514
  39. How eukaryotic transcriptional activators work.
    Nature. 1988 Oct 20;335(6192):683-9 PMID: 3050531
  40. Purification and characterization of proteins that bind to yeast ARSs.
    J Biol Chem. 1988 Nov 25;263(33):17270-7 PMID: 3053706
  41. A comprehensive set of sequence analysis programs for the VAX.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95 PMID: 6546423
  42. Isolation and characterisation of a yeast chromosomal replicator.
    Nature. 1979 Nov 1;282(5734):39-43 PMID: 388229
  43. A yeast silencer contains sequences that can promote autonomous plasmid replication and transcriptional activation.
    Cell. 1987 Dec 4;51(5):709-19 PMID: 3315230
  44. Saturation mutagenesis of the yeast his3 regulatory site: requirements for transcriptional induction and for binding by GCN4 activator protein.
    Science. 1986 Oct 24;234(4775):451-7 PMID: 3532321
  45. The SV40 enhancer can be dissected into multiple segments, each with a different cell type specificity.
    Genes Dev. 1987 Mar;1(1):65-74 PMID: 2828161
  46. Distinguishing between mechanisms of eukaryotic transcriptional activation with bacteriophage T7 RNA polymerase.
    Cell. 1987 Sep 25;50(7):1047-55 PMID: 3304661
  47. Nucleotide sequence of the tcml gene (ribosomal protein L3) of Saccharomyces cerevisiae.
    J Bacteriol. 1983 Jul;155(1):8-14 PMID: 6305925
  48. Effect of ARS1 mutations on chromosome stability in Saccharomyces cerevisiae.
    Mol Cell Biol. 1985 Jul;5(7):1676-84 PMID: 3894934
  49. Regulation of inducible and tissue-specific gene expression.
    Science. 1987 Jun 5;236(4806):1237-45 PMID: 3296191
  50. Transcription initiation of the Saccharomyces cerevisiae iso-1-cytochrome c gene. Multiple, independent T-A-T-A sequences.
    J Mol Biol. 1986 Feb 5;187(3):363-78 PMID: 3009831
  51. A single gene from yeast for both nuclear and cytoplasmic polyadenylate-binding proteins: domain structure and expression.
    Cell. 1986 Jun 20;45(6):827-35 PMID: 3518950
  52. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  53. Sequence-specific interactions between cellular DNA-binding proteins and the adenovirus origin of DNA replication.
    Mol Cell Biol. 1987 Feb;7(2):875-86 PMID: 3821731
  54. Affinity purification of sequence-specific DNA binding proteins.
    Proc Natl Acad Sci U S A. 1986 Aug;83(16):5889-93 PMID: 3461465
  55. Specific DNA binding of GAL4, a positive regulatory protein of yeast.
    Cell. 1985 Apr;40(4):767-74 PMID: 3886158
  56. Measurement of the binding of transcription factor Sp1 to a single GC box recognition sequence.
    Nucleic Acids Res. 1989 Apr 11;17(7):2639-53 PMID: 2717405
  57. Nucleotide sequences of yeast genes for tRNA(2), tRNA(2) and tRNA(1): homology blocks occur in the vicinity of different tRNA genes.
    EMBO J. 1982;1(3):291-5 PMID: 16453416
  58. An upstream signal is required for in vitro transcription of Neurospora 5S RNA genes.
    Mol Gen Genet. 1986 Oct;205(1):189-92 PMID: 3025558
  59. Transformation of intact yeast cells treated with alkali cations.
    J Bacteriol. 1983 Jan;153(1):163-8 PMID: 6336730
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.3.887-897.1990
Published
1990-03-00
Pages
887-97
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360926
Subset
IM
Grants
NIGMS NIH HHS · GM-36659 · United States
External Links
PubMed source DOI: 10.1128/mcb.10.3.887-897.1990 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2