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PMID: 1408796 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Isolation of genomic DNA encoding transcription factor TFIID from Acanthamoeba castellanii: characterization of the promoter.

Nucleic acids research ·Vol. 20 ·No. 18 ·1992-09-25 ·Pages 4817-24

Wong JM, Liu F, Bateman E

Abstract

We have isolated a genomic clone encoding Acanthamoeba castellanii TFIID. The clone contains the entire TFIID gene, 300 bp of 5' promoter sequences and several hundred base pairs of 3' non-coding sequence. The coding region is interrupted by two short introns, but is otherwise identical to Acanthamoeba TFIID cDNA. Comparisons between forty four Acanthamoeba intron 5' and 3' boundaries suggest a 5' splice site consensus of GTACG(T/C) and a 3' consensus of CAG. We determined the position of the transcription initiation site used in vivo, and show that the same site is used in vitro by homologous nuclear extracts. Deletion analysis of the promoter region shows that the minimal promoter required for efficient expression in vitro is located between -97 and +4 relative to the transcription start site. Three regions within the promoter are important for transcription in vitro; sequences between -97 and -35, the TATAAA box and the initiation region. The initiation region is dispensable but appears to position the transcription start site relative to the TATAAA box. The TATAAA box is absolutely required for transcription initiation whereas the upstream region stimulates transcription approximately five-fold.

MeSH Terms
Acanthamoeba/genetics Amino Acid Sequence Animals Base Sequence Cell Nucleus/metabolism DNA/genetics,isolation & purification Exons Genomic Library Introns Molecular Sequence Data Mutagenesis Promoter Regions, Genetic RNA, Messenger/genetics,metabolism Sequence Deletion TATA Box Transcription Factor TFIID Transcription Factors/genetics Transcription, Genetic
Chemicals
RNA, Messenger Transcription Factor TFIID Transcription Factors DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wong J M
Department of Microbiology and Molecular Genetics, University of Vermont, Burlington 05405-0068.
Liu F
Bateman E
References (47)
47 references, click to expand
  1. Cloning of the Schizosaccharomyces pombe TFIID gene reveals a strong conservation of functional domains present in Saccharomyces cerevisiae TFIID.
    Genes Dev. 1990 Jul;4(7):1141-8 PMID: 2210373
  2. Gene transcription. TFIIB or not TFIIB?
    Nature. 1991 May 2;351(6321):16-8 PMID: 2027376
  3. Yeast TATA-binding protein TFIID binds to TATA elements with both consensus and nonconsensus DNA sequences.
    Proc Natl Acad Sci U S A. 1989 Aug;86(15):5718-22 PMID: 2569738
  4. Yeast TATA-box transcription factor gene.
    Proc Natl Acad Sci U S A. 1989 Oct;86(20):7785-9 PMID: 2682626
  5. Five intermediate complexes in transcription initiation by RNA polymerase II.
    Cell. 1989 Feb 24;56(4):549-61 PMID: 2917366
  6. A cellular DNA-binding protein that activates eukaryotic transcription and DNA replication.
    Cell. 1987 Jan 16;48(1):79-89 PMID: 3024847
  7. Positive and negative regulation of transcription in vitro: enhancer-binding protein AP-2 is inhibited by SV40 T antigen.
    Cell. 1987 Sep 11;50(6):847-61 PMID: 3040262
  8. Eukaryotic RNA polymerase I promoter binding is directed by protein contacts with transcription initiation factor and is DNA sequence-independent.
    Cell. 1987 Aug 28;50(5):693-9 PMID: 3113736
  9. A yeast activity can substitute for the HeLa cell TATA box factor.
    Nature. 1988 Jul 7;334(6177):77-80 PMID: 3290688
  10. Physical analysis of transcription preinitiation complex assembly on a class II gene promoter.
    Science. 1988 Sep 9;241(4871):1335-8 PMID: 3413495
  11. Molecular biology of DNA in Acanthamoeba, Amoeba, Entamoeba, and Naegleria.
    Int Rev Cytol. 1986;99:311-41 PMID: 3514511
  12. Regulation of eukaryotic ribosomal RNA transcription by RNA polymerase modification.
    Cell. 1986 Nov 7;47(3):445-50 PMID: 3768960
  13. Interaction of a gene-specific transcription factor with the adenovirus major late promoter upstream of the TATA box region.
    Cell. 1985 Nov;43(1):165-75 PMID: 4075392
  14. Generation and functional analyses for base-substitution mutants of the adenovirus 2 major late promoter.
    Nucleic Acids Res. 1984 Dec 21;12(24):9309-21 PMID: 6514578
  15. Cloning and expression of the Acanthamoeba castellanii gene encoding transcription factor TFIID.
    Gene. 1992 Aug 1;117(1):91-7 PMID: 1339370
  16. TATA-binding protein is a classless factor.
    Cell. 1992 Mar 6;68(5):819-21 PMID: 1547484
  17. The TATA-binding protein and associated factors are integral components of the RNA polymerase I transcription factor, SL1.
    Cell. 1992 Mar 6;68(5):965-76 PMID: 1547496
  18. Variants of the TATA-binding protein can distinguish subsets of RNA polymerase I, II, and III promoters.
    Cell. 1992 May 15;69(4):697-702 PMID: 1586948
  19. YY1 is an initiator sequence-binding protein that directs and activates transcription in vitro.
    Nature. 1991 Nov 21;354(6350):241-5 PMID: 1720509
  20. TFIID binds in the minor groove of the TATA box.
    Cell. 1991 Dec 20;67(6):1231-40 PMID: 1760847
  21. Mechanism of action of an acidic transcriptional activator in vitro.
    Cell. 1991 Mar 8;64(5):971-81 PMID: 2001592
  22. Commitment and activation at pol II promoters: a tail of protein-protein interactions.
    Cell. 1990 Jun 29;61(7):1161-4 PMID: 2194664
  23. RNA polymerase B (II) and general transcription factors.
    Annu Rev Biochem. 1990;59:711-54 PMID: 2197989
  24. The "initiator" as a transcription control element.
    Cell. 1989 Apr 7;57(1):103-13 PMID: 2467742
  25. Myosin I heavy-chain genes of Acanthamoeba castellanii: cloning of a second gene and evidence for the existence of a third isoform.
    Gene. 1989 Oct 30;82(2):269-80 PMID: 2511079
  26. Promoter specificity and modulation of RNA polymerase II transcription.
    FASEB J. 1989 Apr;3(6):1723-33 PMID: 2649403
  27. Cloning and structure of a yeast gene encoding a general transcription initiation factor TFIID that binds to the TATA box.
    Nature. 1989 Sep 28;341(6240):299-303 PMID: 2677740
  28. Promoters, activator proteins, and the mechanism of transcriptional initiation in yeast.
    Cell. 1987 May 8;49(3):295-7 PMID: 2882858
  29. Lambda ZAP: a bacteriophage lambda expression vector with in vivo excision properties.
    Nucleic Acids Res. 1988 Aug 11;16(15):7583-600 PMID: 2970625
  30. Fission yeast Schizosaccharomyces pombe correctly excises a mammalian RNA transcript intervening sequence.
    Nature. 1985 Nov 7-13;318(6041):78-80 PMID: 2997624
  31. Purified transcription factor AP-1 interacts with TPA-inducible enhancer elements.
    Cell. 1987 Jun 19;49(6):741-52 PMID: 3034433
  32. Complete nucleotide sequence and deduced polypeptide sequence of a nonmuscle myosin heavy chain gene from Acanthamoeba: evidence of a hinge in the rodlike tail.
    J Cell Biol. 1987 Aug;105(2):913-25 PMID: 3040773
  33. Events during eucaryotic rRNA transcription initiation and elongation: conversion from the closed to the open promoter complex requires nucleotide substrates.
    Mol Cell Biol. 1988 May;8(5):1940-6 PMID: 3133551
  34. Function of a yeast TATA element-binding protein in a mammalian transcription system.
    Nature. 1988 Jul 7;334(6177):37-42 PMID: 3290687
  35. Regulation of inducible and tissue-specific gene expression.
    Science. 1987 Jun 5;236(4806):1237-45 PMID: 3296191
  36. Fine structure genetic analysis of a beta-globin promoter.
    Science. 1986 May 2;232(4750):613-8 PMID: 3457470
  37. The heavy chain of Acanthamoeba myosin IB is a fusion of myosin-like and non-myosin-like sequences.
    Proc Natl Acad Sci U S A. 1987 Oct;84(19):6720-4 PMID: 3477803
  38. Transcriptional selectivity of viral genes in mammalian cells.
    Cell. 1986 Sep 12;46(6):795-805 PMID: 3530495
  39. Isolation of a non-muscle myosin heavy chain gene from Acanthamoeba.
    J Biol Chem. 1986 Feb 5;261(4):1949-56 PMID: 3944121
  40. Transcriptional control signals of a eukaryotic protein-coding gene.
    Science. 1982 Jul 23;217(4557):316-24 PMID: 6283634
  41. Actin genes and actin messenger RNA in Acanthamoeba castellanii. Nucleotide sequence of the split actin gene I.
    J Mol Biol. 1982 Jul 25;159(1):1-18 PMID: 6290670
  42. Organization and expression of eucaryotic split genes coding for proteins.
    Annu Rev Biochem. 1981;50:349-83 PMID: 6791577
  43. Growth, reproduction, and differentiation in Acanthamoeba.
    Int Rev Cytol. 1979;61:283-338 PMID: 389870
  44. The TATA-binding protein is required for transcription by all three nuclear RNA polymerases in yeast cells.
    Cell. 1992 May 15;69(4):685-96 PMID: 1586947
  45. Transcription from a TATA-less promoter requires a multisubunit TFIID complex.
    Genes Dev. 1991 Nov;5(11):1935-45 PMID: 1657708
  46. Interaction of TFIID in the minor groove of the TATA element.
    Cell. 1991 Dec 20;67(6):1241-50 PMID: 1760848
  47. Stability of transcription complexes on class II genes.
    Mol Cell Biol. 1989 Jan;9(1):342-4 PMID: 2494433
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/20.18.4817
Published
1992-09-25
Pages
4817-24
Language
English
Region
England
NLM ID
0411011
PMCID
PMC334237
Subset
IM
Grants
NEI NIH HHS · EY 08706 · United States
Databases
GENBANK
M93340
External Links
PubMed source DOI: 10.1093/nar/20.18.4817 PMC Full Text View journal details
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