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PMID: 1657708 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Transcription from a TATA-less promoter requires a multisubunit TFIID complex.

Genes & development ·Vol. 5 ·No. 11 ·1991-11-00 ·Pages 1935-45

Pugh BF, Tjian R

Abstract

In eukaryotes, the TATA box-binding protein (TBP) is responsible for nucleating assembly of the transcription initiation machinery. Here, we report that a TFIID complex containing TBP is essential for transcription even at a promoter that lacks a TATA box. Immunopurification of TFIID reveals that the active species in reconstituting TATA-less transcription is a multisubunit complex consisting of TBP and many TBP-associated factors (TAFs).

MeSH Terms

Animals Base Sequence Blotting, Western Drosophila/genetics Gene Expression Regulation/genetics HeLa Cells Humans Macromolecular Substances Molecular Sequence Data Promoter Regions, Genetic/genetics Recombinant Proteins/genetics,isolation & purification,metabolism Simian virus 40/genetics Sp1 Transcription Factor/genetics TATA Box/genetics Transcription Factor TFIID Transcription Factors/antagonists & inhibitors,genetics,metabolism Transcription, Genetic/genetics

Chemicals

Macromolecular Substances Recombinant Proteins Sp1 Transcription Factor Transcription Factor TFIID Transcription Factors

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Pugh B F

Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720.

Tjian R

Article Info

Journal

Genes & development

Abbr.

Genes Dev

ISSN

0890-9369

DOI

10.1101/gad.5.11.1935

Published

1991-11-00

Pages

1935-45

Language

English

Region

United States

NLM ID

8711660

Subset

External Links

PubMed source DOI: 10.1101/gad.5.11.1935 View journal details

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Transcription from a TATA-less promoter requires a multisubunit TFIID complex.

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