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PMID: 2569738 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Yeast TATA-binding protein TFIID binds to TATA elements with both consensus and nonconsensus DNA sequences.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 15 ·1989-08-00 ·Pages 5718-22

Hahn S, Buratowski S, Sharp PA, Guarente L

Abstract

The DNA binding properties of the yeast TATA element-binding protein TFIID were investigated. The affinity (apparent equilibrium dissociation constant) of TFIID for the adenovirus major late promoter consensus TATA element is 2 x 10(-9) M, a value similar to the affinity of gene-specific regulatory proteins for their binding sites. TFIID binding is highly specific and recognizes nonspecific sites with approximately 10(5)-fold lower affinity. Despite this specificity, TFIID also binds with high affinity to several TATA elements that do not match the consensus TATA sequences (TATAAA and TATATA): the yeast LEU2 TATA (TATTATTTA), the simian virus 40 TATA (CTTATTTAT), and the yeast CYC1 -10 TATA (TTATACATT) all bound TFIID. Furthermore, TFIID was active in promoting transcription in vitro from the nonconsensus TATA elements. Thus, contrary to previous suggestions, the existence of nonconsensus TATA elements does not itself indicate the existence of multiple TATA-binding factors.

MeSH Terms
Base Sequence DNA/metabolism Deoxyribonuclease I Genes, Homeobox Kinetics Molecular Sequence Data Protein Binding Saccharomyces cerevisiae/genetics,metabolism Structure-Activity Relationship Transcription Factor TFIID Transcription Factors/metabolism
Chemicals
Transcription Factor TFIID Transcription Factors DNA Deoxyribonuclease I
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hahn S
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
Buratowski S
Sharp P A
Guarente L
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.15.5718
Published
1989-08-00
Pages
5718-22
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC297701
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.86.15.5718 PMC Full Text View journal details
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