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PMID: 8414988 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

An upstream promoter element of the Acanthamoeba castellanii TBP gene binds a DNA sequence specific transcription activating protein, TPBF.

Nucleic acids research ·Vol. 21 ·No. 18 ·1993-09-11 ·Pages 4321-9

Liu F, Bateman E

Abstract

We have characterized a positive-acting element in the upstream portion of the Acanthamoeba TBP gene promoter. The 27 bp element (TPE), located within the promoter between -97 and -70, stimulates transcription in an orientation independent fashion and tolerates modest changes in its distance from the TATA box. The TPE does not, however, function synergistically nor when positioned 3000 bp 5' or 260 base pairs 3' of the transcription start site. The TPE binds a DNA sequence-dependent factor, TPBF, which we have partly purified. TPBF was characterized using in vitro transcription, DNase I footprinting, methylation interference and electrophoretic mobility shift assays. TPBF does not have a counterpart in HeLa cells, but nonetheless strongly stimulates transcription of the Acanthamoeba TBP gene in mammalian extracts. Our results also suggest that there are additional positively and negatively acting elements within the TBP gene promoter, for which a model is presented.

Related Genes
TBP
MeSH Terms
Acanthamoeba/genetics,metabolism Animals Base Sequence DNA/metabolism HeLa Cells Humans Molecular Sequence Data Oligodeoxyribonucleotides Promoter Regions, Genetic Sequence Deletion Templates, Genetic Trans-Activators/metabolism Transcription Factor TFIID Transcription Factors/genetics Transcription, Genetic
Chemicals
Oligodeoxyribonucleotides Trans-Activators Transcription Factor TFIID Transcription Factors DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Liu F
Department of Microbiology and Molecular Genetics, University of Vermont, Burlington 05405-0068.
Bateman E
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/21.18.4321
Published
1993-09-11
Pages
4321-9
Language
English
Region
England
NLM ID
0411011
PMCID
PMC310067
Subset
IM
Grants
NEI NIH HHS · EY 08706 · United States
External Links
PubMed source DOI: 10.1093/nar/21.18.4321 PMC Full Text View journal details
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