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PMID: 2187179 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Yeast general transcription factor GFI: sequence requirements for binding to DNA and evolutionary conservation.

Nucleic acids research ·Vol. 18 ·No. 9 ·1990-05-11 ·Pages 2769-76

Dorsman JC, van Heeswijk WC, Grivell LA

Abstract

GFI is an abundant DNA binding protein in the yeast S. cerevisiae. The protein binds to specific sequences in both ARS elements and the upstream regions of a large number of genes and is likely to play an important role in yeast cell growth. To get insight into the relative strength of the various GFI-DNA binding sites within the yeast genome, we have determined dissociation rates for several GFI-DNA complexes and found them to vary over a 70-fold range. Strong binding sites for GFI are present in the upstream activating sequences of the gene encoding the 40 kDa subunit II of the QH2:cytochrome c reductase, the gene encoding ribosomal protein S33 and in the intron of the actin gene. The binding site in the ARS1-TRP1 region is of intermediate strength. All strong binding sites conform to the sequence 5' RTCRYYYNNNACG-3'. Modification interference experiments and studies with mutant binding sites indicate that critical bases for GFI recognition are within the two elements of the consensus DNA recognition sequence. Proteins with the DNA binding specificities of GFI and GFII can also be detected in the yeast K. lactis, suggesting evolutionary conservation of at least the respective DNA-binding domains in both yeasts.

MeSH Terms
Base Sequence Binding Sites Binding, Competitive Biological Evolution DNA, Circular/metabolism DNA, Fungal/genetics,metabolism DNA, Single-Stranded/metabolism DNA-Binding Proteins/metabolism Kluyveromyces/genetics Molecular Sequence Data Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Transcription Factors/metabolism
Chemicals
ABFI protein, S cerevisiae DNA, Circular DNA, Fungal DNA, Single-Stranded DNA-Binding Proteins Saccharomyces cerevisiae Proteins Transcription Factors
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Dorsman J C
Department of Molecular Cell Biology, University of Amsterdam, The Netherlands.
van Heeswijk W C
Grivell L A
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/18.9.2769
Published
1990-05-11
Pages
2769-76
Language
English
Region
England
NLM ID
0411011
PMCID
PMC330762
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/18.9.2769 PMC Full Text View journal details
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