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PMID: 8760868 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Recruitment of damaged DNA to the nuclear matrix in hamster cells following ultraviolet irradiation.

Nucleic acids research ·Vol. 24 ·No. 15 ·1996-08-01 ·Pages 2877-84

Koehler DR, Hanawalt PC

Abstract

We examined the relationship between the nuclear matrix and DNA in the dihydrofolate reductase domain following irradiation of Chinese hamster cells with UV light. The fraction of matrix-bound DNA increased in transcribed and non-transcribed regions during a 3 h period after irradiation. However, no increase was observed with excision repair-deficient cells mutant for the ERCC1 gene. The major UV-induced lesion, the cyclobutane pyrimidine dimer, increased in frequency in the matrix-bound DNA 1 h after irradiation, in both transcribed and non-transcribed regions, but decreased subsequently. This phenomenon was also lacking in excision repair-deficient cells. These data demonstrate that recruitment of lesion-containing DNA to the nuclear matrix occurs following UV irradiation and suggest that this recruitment is dependent upon nucleotide excision repair. This is consistent with the concept of a 'repair factory' residing on the nuclear matrix at which excision repair occurs.

MeSH Terms
Animals CHO Cells Cell Compartmentation Cricetinae DNA Damage DNA Repair Nuclear Matrix/metabolism Subcellular Fractions Tetrahydrofolate Dehydrogenase/metabolism Ultraviolet Rays/adverse effects
Chemicals
Tetrahydrofolate Dehydrogenase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Koehler D R
Department of Biological Sciences, Stanford University, CA 94305-5020, USA.
Hanawalt P C
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/24.15.2877
Published
1996-08-01
Pages
2877-84
Language
English
Region
England
NLM ID
0411011
PMCID
PMC146037
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/24.15.2877 PMC Full Text View journal details
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