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PMID: 2414739 Published · ppublish English Journal Article

Low ionic strength extraction of nuclease-treated nuclei destroys the attachment of transcriptionally active DNA to the nuclear skeleton.

Nucleic acids research ·Vol. 13 ·No. 20 ·1985-10-25 ·Pages 7427-44

Razin SV, Yarovaya OV, Georgiev GP

Abstract

We have studied how the conditions in which the nuclear matrix is isolated influence the association of transcribing DNA with the nuclear matrix. Extraction of nuclease-treated nuclei with a low ionic strength solution before a high salt nuclei with a low ionic strength solution before a high salt extraction completely abolishes this association. However, RNA removal by RNAase treatment does not affect the binding of transcribing DNA to the nuclear matrix. The nature of the association of active genes with the nuclear matrix is discussed.

MeSH Terms
Animals Cell Fractionation/methods Cell Nucleus/metabolism,ultrastructure Cells, Cultured Copper/pharmacology DNA/metabolism Deoxyribonucleases Gene Expression Regulation Immunoglobulin mu-Chains/genetics Kinetics Mice Osmolar Concentration RNA/metabolism RNA Polymerase II/metabolism Ribonucleases Solubility Transcription, Genetic
Chemicals
Immunoglobulin mu-Chains RNA Copper DNA RNA Polymerase II Deoxyribonucleases Ribonucleases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Razin S V
Yarovaya O V
Georgiev G P
References (23)
23 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.20.7427
Published
1985-10-25
Pages
7427-44
Language
English
Region
England
NLM ID
0411011
PMCID
PMC322053
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/13.20.7427 PMC Full Text View journal details
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