Home LiteratureArticle Details
PMID: 6329681 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Active viral genes in transformed cells lie close to the nuclear cage.

The EMBO journal ·Vol. 1 ·No. 4 ·1982-00-00 ·Pages 447-52

Cook PR, Lang J, Hayday A, Lania L, Fried M, Chiswell DJ, Wyke JA

Abstract

Nuclear DNA is looped by attachment to a matrix or cage. Using nine different lines transformed by polyoma or avian sarcoma virus, we have mapped viral sequences integrated within these loops. In all lines that contain high concentrations of viral transcripts and express the transformed phenotype, the integrated viral genes lie close to the points of attachment to the cage. Integration of polyoma DNA induces outlying cellular sequences to become closely associated with the cage. The strength of this correlation between gene activity and proximity to the cage was examined using sub-clones of one avian sarcoma virus transformant. Proviral sequences are closely associated with the cage in this transformant, much less so in two untransformed 'flat revertants' which contain no detectable viral transcripts but regain their close association with the cage in two retransformed derivatives.

MeSH Terms
Animals Avian Sarcoma Viruses/genetics Base Sequence Cell Line Cell Nucleus/analysis Cell Transformation, Viral DNA, Viral/analysis Genes, Viral Polyomavirus/genetics Rats Serum Albumin/genetics Transcription, Genetic
Chemicals
DNA, Viral Serum Albumin
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Cook P R
Lang J
Hayday A
Lania L
Fried M
Chiswell D J
Wyke J A
References (40)
40 references, click to expand
  1. The SV40 72 base repair repeat has a striking effect on gene expression both in SV40 and other chimeric recombinants.
    Nucleic Acids Res. 1981 Nov 25;9(22):6047-68 PMID: 6273820
  2. Characterization of nuclear structures containing superhelical DNA.
    J Cell Sci. 1976 Nov;22(2):303-24 PMID: 1002771
  3. A small segment of polyoma virus DNA enhances the expression of a cloned beta-globin gene over a distance of 1400 base pairs.
    Nucleic Acids Res. 1981 Dec 11;9(23):6251-64 PMID: 6275354
  4. Multiple arrangements of viral DNA and an activated host oncogene in bursal lymphomas.
    Nature. 1982 Jan 21;295(5846):209-14 PMID: 6276760
  5. Activation of SV40 genome by 72-base pair tandem repeats of Moloney sarcoma virus.
    Nature. 1982 Feb 18;295(5850):568-72 PMID: 6276775
  6. Molecular mechanisms involved in morphological variation of avian sarcoma virus-infected rat cells.
    Virology. 1982 Jan 30;116(2):428-40 PMID: 6278722
  7. The structure of histone-depleted metaphase chromosomes.
    Cell. 1977 Nov;12(3):817-28 PMID: 922894
  8. Supercoiling of DNA and nuclear conformation during the cell-cycle.
    J Cell Sci. 1978 Apr;30:211-26 PMID: 649687
  9. Domains in chromatin structure.
    Cold Spring Harb Symp Quant Biol. 1978;42 Pt 1:109-18 PMID: 277332
  10. Proviruses of avian sarcoma virus are terminally redundant, co-extensive with unintegrated linear DNA and integrated at many sites.
    Cell. 1978 Dec;15(4):1397-410 PMID: 215325
  11. Purification of specific adenovirus 2 RNAs by preparative hybridization and selective thermal elution.
    Nucleic Acids Res. 1979 Feb;6(2):593-607 PMID: 424306
  12. A partial characterization of DNA fragments protected from nuclease degradation in histone depleted metaphase chromosomes of the Chinese hamster.
    Nucleic Acids Res. 1979 Sep 11;7(1):49-67 PMID: 493144
  13. The rat serum albumin gene: analysis of cloned sequences.
    Proc Natl Acad Sci U S A. 1979 Jul;76(7):3256-60 PMID: 158758
  14. The similarity of DNA sequences remaining bound to scaffold upon nuclease treatment of interphase nuclei and metaphase chromosomes.
    Nucleic Acids Res. 1979 Nov 24;7(6):1713-35 PMID: 503867
  15. Electron-microscopy of intact nuclear DNA from human cells.
    J Cell Sci. 1979 Oct;39:53-62 PMID: 231042
  16. The polyoma virus 100K large T-antigen is not required for the maintenance of transformation.
    Virology. 1980 Feb;101(1):217-32 PMID: 6243821
  17. Sequence analysis of nuclear matrix associated DNA from rat liver.
    Exp Cell Res. 1980 Aug;128(2):466-70 PMID: 7409002
  18. Association of polyoma T antigen and DNA with the nuclear matrix from lytically infected 3T6 cells.
    Cell. 1980 Nov;22(1 Pt 1):37-46 PMID: 6253084
  19. Organization and expression of integrated polyoma virus DNA sequences in transformed rodent cells.
    Cold Spring Harb Symp Quant Biol. 1980;44 Pt 1,:597-603 PMID: 6253160
  20. Comparison of polyoma virus transcription in productively infected mouse cells and transformed rodent cell lines.
    Cold Spring Harb Symp Quant Biol. 1980;44 Pt 1,:63-75 PMID: 6253165
  21. Mapping sequences in loops of nuclear DNA by their progressive detachment from the nuclear cage.
    Nucleic Acids Res. 1980 Jul 11;8(13):2895-906 PMID: 7433096
  22. Infection of rat cells by avian sarcoma virus: factors affecting transformation and subsequent reversion.
    Virology. 1980 Oct 30;106(2):217-33 PMID: 6254248
  23. Nuclear non-chromatin proteinaceous structures: their role in the organization and function of the interphase nucleus.
    J Cell Sci. 1980 Aug;44:395-435 PMID: 7002943
  24. Molecular cloning and characterization of avian sarcoma virus circular DNA molecules.
    J Virol. 1980 Oct;36(1):50-61 PMID: 6255214
  25. The evolution of alpha-fetoprotein and albumin. II. The structures of the alpha-fetoprotein and albumin genes in the mouse.
    J Biol Chem. 1981 Feb 25;256(4):1960-7 PMID: 6161930
  26. Localization of SV40 genes within supercoiled loop domains.
    Nucleic Acids Res. 1980 Dec 11;8(23):5623-33 PMID: 6258148
  27. DNA is replicated at the nuclear cage.
    J Cell Sci. 1980 Dec;46:365-86 PMID: 7228913
  28. 5-Methylcytosine in eukaryotic DNA.
    Science. 1981 Jun 19;212(4501):1350-7 PMID: 6262918
  29. RNA is synthesized at the nuclear cage.
    Nature. 1981 Aug 6;292(5823):552-5 PMID: 6166873
  30. Enrichment of satellite DNA on the nuclear matrix of bovine cells.
    Nature. 1981 Dec 3;294(5840):481-2 PMID: 7312040
  31. Hybridization of nuclear matrix attached deoxyribonucleic acid fragments.
    Biochemistry. 1981 Nov 24;20(24):6921-9 PMID: 7317362
  32. The ovalbumin gene is associated with the nuclear matrix of chicken oviduct cells.
    Cell. 1982 Jan;28(1):99-106 PMID: 7066988
  33. Effect of 3-methylcholanthrene and dimethylnitrosamine on anchorage dependence of rat fibroblasts chronically infected with Rauscher leukemia virus.
    Int J Cancer. 1973 Jan 15;11(1):123-30 PMID: 4790831
  34. Identification of a nuclear protein matrix.
    Biochem Biophys Res Commun. 1974 Oct 23;60(4):1410-7 PMID: 4214419
  35. Structure, biochemistry, and functions of the nuclear envelope.
    Int Rev Cytol. 1974;Suppl 4:71-236 PMID: 4616920
  36. Isolation of nuclear pore complexes in association with a lamina.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):1007-11 PMID: 1055359
  37. The isolation and characterization of nuclear ghosts from cultured HeLa cells.
    Biochemistry. 1975 Jul;14(13):3005-13 PMID: 1096936
  38. Supercoils in human DNA.
    J Cell Sci. 1975 Nov;19(2):261-79 PMID: 1202042
  39. Chromosomal subunits in active genes have an altered conformation.
    Science. 1976 Sep 3;193(4256):848-56 PMID: 948749
  40. DNA fragments associated with chromosome scaffolds.
    Nucleic Acids Res. 1981 Oct 10;9(19):5093-108 PMID: 6273794
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1982-00-00
Pages
447-52
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553066
Subset
IM
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2