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PMID: 6091035 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Attachment of repeated sequences to the nuclear cage.

Nucleic acids research ·Vol. 12 ·No. 17 ·1984-09-11 ·Pages 6709-26

Jackson DA, Cook PR, Patel SB

Abstract

Nuclear DNA is probably organized into loops by attachment to a sub-structure in vivo. When HeLa cells are lysed in Triton and 2M NaCl the resulting nucleoids contain naked DNA which is supercoiled so the loops must remain intact. We have attempted to identify sequences responsible for attaching these loops to the nuclear sub-structure by progressively detaching DNA with various nucleases. Fragments at the 5' end of the ribosomal RNA locus, and a variety of transcribed and repeated sequences, are shown to lie relatively close to attachment points. This implies that sequences cannot be arranged randomly. However no "attachment sequence" could be identified.

MeSH Terms
Base Sequence Cell Fractionation Cell Nucleus/analysis DNA Restriction Enzymes DNA, Neoplasm/isolation & purification DNA, Ribosomal/genetics HeLa Cells/analysis Humans Nucleic Acid Hybridization RNA, Ribosomal/genetics Repetitive Sequences, Nucleic Acid
Chemicals
DNA, Neoplasm DNA, Ribosomal RNA, Ribosomal DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Jackson D A
Cook P R
Patel S B
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35 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.17.6709
Published
1984-09-11
Pages
6709-26
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320111
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/12.17.6709 PMC Full Text View journal details
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