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PMID: 2398893 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Mapping of structural and transcription-related matrix attachment sites in the alpha-globin gene domain of avian erythroblasts and erythrocytes.

Molecular and cellular biology ·Vol. 10 ·No. 10 ·1990-10-00 ·Pages 5349-58

Farache G, Razin SV, Rzeszowska-Wolny J, Moreau J, Targa FR, Scherrer K

Abstract

The positions of preferential DNA interaction with the nuclear matrix were mapped within the domain of the chicken alpha-globin genes in transcriptionally active erythroblast nuclei and inactive nuclei of mature erythrocytes. In the latter, only two major distinct attachment sites were observed, close to the A + T-rich sequences previously found at the boundaries of the domain. Sequencing of these structural matrix attachment points revealed several known DNA motifs; some of them were present on both sides of the domain. In actively transcribing erythroblast nuclei of adult animals, a large fraction of the transcribed area was represented in nuclear matrix DNA, including upstream and downstream elements. In particular, adult alpha A- and alpha D-globin genes were found in matrix DNA, while the transcribed but translationally unexpressed embryonic pi gene was underrepresented. The data are discussed in terms of the existence of stable or structural and expression-related matrix attachment sites; correlations to the origin of replication and the units of transcription of the domain are shown.

MeSH Terms
Animals Base Sequence Chickens Cloning, Molecular Erythrocytes/physiology,ultrastructure Gene Expression Globins/genetics Microscopy, Electron Molecular Sequence Data Nuclear Matrix/ultrastructure Nucleic Acid Hybridization Regulatory Sequences, Nucleic Acid Sequence Homology, Nucleic Acid Transcription, Genetic
Chemicals
Globins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Farache G
Institut Jacques Monod, Université Paris.
Razin S V
Rzeszowska-Wolny J
Moreau J
Targa F R
Scherrer K
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.10.5349-5358.1990
Published
1990-10-00
Pages
5349-58
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC361231
Subset
IM
Databases
GENBANK
M34465, M58748, M58749, X51600
External Links
PubMed source DOI: 10.1128/mcb.10.10.5349-5358.1990 PMC Full Text View journal details
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