Replication origins are attached to the nuclear skeleton.

Nucleic acids research ·Vol. 14 ·No. 20 ·1986-10-24 ·Pages 8189-207

Razin SV, Kekelidze MG, Lukanidin EM, Scherrer K, Georgiev GP

Abstract

DNA fragments containing replication origins (oriDNA) were isolated from a chicken erythroblast cell line by a modified procedure of Zannis-Hadjopoulos et al. and studied in the renaturation reaction driven by either total or nuclear matrix DNA (nmDNA) from the same cells or from mature erythrocytes. We found that the unique sequences of nmDNA from erythroblasts (5 kb long) represented a specific subset of sequences constituting about a quarter of total DNA unique sequences, while the erythrocyte nmDNA 5 kb fragments constitute only about one tenth of total unique DNA and all are recovered among erythroblast nmDNA. Virtually all oriDNA sequences are present in the fraction of erythrocyte nmDNA. Thereafter, the putative positions of replication origins within the alpha-globine gene domain have been mapped by hybridization experiments. They were found to coincide with the previously established positions of permanent sites of DNA attachment to the nuclear matrix.

MeSH Terms

Animals Cell Fractionation Cell Line Cell Nucleus/physiology,ultrastructure Chickens Chromatin/ultrastructure Chromosome Mapping DNA/physiology DNA Replication Globins/genetics

Chemicals

Chromatin Globins DNA

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Razin S V

Kekelidze M G

Lukanidin E M

Scherrer K

Georgiev G P

References (20)

20 references, click to expand

[Localization of regions of DNA attachment to the nuclear skeleton within chicken alpha-globin genes in functionally active and functionally inactive nuclei].
Mol Biol (Mosk). 1985 Mar-Apr;19(2):456-66 PMID: 2987668
The similarity of DNA sequences remaining bound to scaffold upon nuclease treatment of interphase nuclei and metaphase chromosomes.
Nucleic Acids Res. 1979 Nov 24;7(6):1713-35 PMID: 503867
The association of transcribed genes with the nuclear matrix of Drosophila cells during heat shock.
Nucleic Acids Res. 1985 Apr 11;13(7):2413-31 PMID: 2987852
Identification of a nuclear protein matrix.
Biochem Biophys Res Commun. 1974 Oct 23;60(4):1410-7 PMID: 4214419
Lesions induced in DNA by ultraviolet light are repaired at the nuclear cage.
J Cell Sci. 1984 Aug;70:189-96 PMID: 6501435
The spatial organization of sequences involved in initiation and termination of eukaryotic DNA replication.
Nucleic Acids Res. 1984 Jan 25;12(2):1069-75 PMID: 6694907
Effects of psoralen on replicon size and mean rate of DNA synthesis in partially synchronized cells of Pisum sativum L.
Exp Cell Res. 1985 Jun;158(2):500-8 PMID: 4007063
Attachment of origins of replication to the nuclear matrix and the chromosomal scaffold.
FEBS Lett. 1984 Jun 4;171(1):13-6 PMID: 6723972
Organization of the higher-order chromatin loop: specific DNA attachment sites on nuclear scaffold.
Cell. 1984 Nov;39(1):223-32 PMID: 6091913
Nuclear matrix. Isolation and characterization of a framework structure from rat liver nuclei.
J Cell Biol. 1977 Jun;73(3):616-37 PMID: 873992
The remarkable instability of replication loops provides a general method for the isolation of origins of DNA replication.
Cell. 1981 Nov;27(1 Pt 2):155-63 PMID: 6276021
Organization of DNA replication in Physarum polycephalum. Attachment of origins of replicons and replication forks to the nuclear matrix.
Nucleic Acids Res. 1983 Feb 25;11(4):1181-95 PMID: 6828380
Newly-initiated DNA isolated from Physarum in early S phase consists of nascent-nascent duplexes.
Exp Cell Res. 1984 Oct;154(2):394-401 PMID: 6383853
Supercoiling of DNA and nuclear conformation during the cell-cycle.
J Cell Sci. 1978 Apr;30:211-26 PMID: 649687
Mapping of the 3'-end positions of simian virus 40 nascent strands.
J Mol Biol. 1983 Apr 25;165(4):599-607 PMID: 6304319
RNA is synthesized at the nuclear cage.
Nature. 1981 Aug 6;292(5823):552-5 PMID: 6166873
Isolation of a DNA fraction from Ehrlich ascites tumour cells containing the putative origin of replication.
J Mol Biol. 1982 Oct 15;161(1):77-87 PMID: 6891405
The organisation of chromatin loops: characterization of a scaffold attachment site.
EMBO J. 1986 Mar;5(3):511-8 PMID: 16453673
Low ionic strength extraction of nuclease-treated nuclei destroys the attachment of transcriptionally active DNA to the nuclear skeleton.
Nucleic Acids Res. 1985 Oct 25;13(20):7427-44 PMID: 2414739
Analysis of the closely linked adult chicken alpha-globin genes in recombinant DNAs.
Proc Natl Acad Sci U S A. 1980 May;77(5):2596-600 PMID: 6248855

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/14.20.8189

Published

1986-10-24

Pages

8189-207

Language

English

Region

England

NLM ID

0411011

PMCID

PMC311844

Subset

External Links

PubMed source DOI: 10.1093/nar/14.20.8189 PMC Full Text View journal details

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