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PMID: 2987966 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

In situ localization of DNA topoisomerase II, a major polypeptide component of the Drosophila nuclear matrix fraction.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 12 ·1985-06-00 ·Pages 4142-6

Berrios M, Osheroff N, Fisher PA

Abstract

DNA topoisomerase II has been immunochemically identified on protein blots as a major polypeptide component of the Drosophila nuclear matrix-pore complex-lamina fraction. Indirect immunofluorescence analyses of larval cryosections have confirmed the nuclear localization of topoisomerase II in situ. Although apparently excluded from the nucleolus, the topoisomerase protein is otherwise distributed throughout the interior of interphase nuclei. Similar immunocytochemical studies performed with permeabilized whole giant cells from third-instar larval salivary glands have shown topoisomerase II to be largely restricted to the polytene chromosomes. Upon nuclear disassembly during mitosis, the topoisomerase polypeptide appears to redistribute diffusely throughout the cell. Faint immunofluorescent staining of mitotic chromosomes is also observed.

MeSH Terms
Animals Cell Nucleus/enzymology,ultrastructure DNA Topoisomerases, Type I/metabolism Drosophila melanogaster/ultrastructure Fluorescent Antibody Technique Immunosorbent Techniques Mitosis Molecular Weight Salivary Glands/ultrastructure
Chemicals
DNA Topoisomerases, Type I
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Berrios M
Osheroff N
Fisher P A
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38 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.12.4142
Published
1985-06-00
Pages
4142-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC397951
Subset
IM
Grants
NIGMS NIH HHS · GM-33132 · United States
NIGMS NIH HHS · GM-33944 · United States
External Links
PubMed source DOI: 10.1073/pnas.82.12.4142 PMC Full Text View journal details
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