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PMID: 8631712 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Kinetics of pyrimidine(6-4)pyrimidone photoproduct repair in Escherichia coli.

Journal of bacteriology ·Vol. 178 ·No. 5 ·1996-03-00 ·Pages 1347-50

Koehler DR, Courcelle J, Hanawalt PC

Abstract

We compared the removal of pyrimidine(6-4)pyrimidone photoproducts [(6-4) photoproducts] and cyclobutane pyrimidine dimers (CPDs) from the genome of repair-proficient Escherichia coli, using monoclonal antibodies specific for each type of lesion. We found that (6-4) photoproducts were removed at a higher rate than CPDs in the first 30 min following a moderate UV dose (40 J/m2). The difference in rates was less than that typically reported for cultured mammalian cells, in which the removal of (6-4) photoproducts is far more rapid than that of CPDs.

MeSH Terms
DNA Damage DNA Repair Dose-Response Relationship, Radiation Escherichia coli/genetics,radiation effects Kinetics Pyrimidine Dimers/immunology,metabolism Ultraviolet Rays
Chemicals
Pyrimidine Dimers pyrimidine-pyrimidone dimer
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Koehler D R
Department of Biological Sciences, Stanford University, California 94305-5020, USA.
Courcelle J
Hanawalt P C
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.178.5.1347-1350.1996
Published
1996-03-00
Pages
1347-50
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC177809
Subset
IM
Grants
NIA NIH HHS · AG-02908 · United States
NCI NIH HHS · CA44349 · United States
External Links
PubMed source DOI: 10.1128/jb.178.5.1347-1350.1996 PMC Full Text View journal details
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