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PMID: 8468343 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Association of individual hnRNP proteins and snRNPs with nascent transcripts.

The Journal of cell biology ·Vol. 121 ·No. 2 ·1993-04-00 ·Pages 219-28

Matunis EL, Matunis MJ, Dreyfuss G

Abstract

As they are transcribed, RNA polymerase II transcripts (hnRNAs or pre-mRNAs) associate with hnRNP proteins and snRNP particles, and the processing of pre-mRNA occurs within these ribonucleoprotein complexes. To better understand the relationship between hnRNP proteins and snRNP particles and their roles in mRNA formation, we have visualized them as they associate with nascent transcripts on the polytene chromosomes of Drosophila melanogaster salivary glands. Simultaneous pairwise detection of the abundant hnRNP proteins hrp36, hrp40, and hrp48 by direct double-label immunofluorescence microscopy reveals all of these proteins are bound to most transcripts, but their relative amounts on different transcripts are not fixed. Numerous differences in the relative amounts of snRNP particles and hnRNP proteins on nascent transcripts are also observed. These observations directly demonstrate that individual hnRNP proteins and snRNP particles are differentially associated with nascent transcripts and suggest that different pre-mRNAs bind different combinations of these factors to form transcript-specific, rather than a single type of, hnRNA-hnRNP-snRNP complexes. The distinct and specific constellation of hnRNP proteins and snRNP particles that assembles on different pre-mRNAs is likely to affect the fate and pathway of processing of these transcripts.

MeSH Terms
Animals Chromosomes Drosophila melanogaster/genetics Heterogeneous-Nuclear Ribonucleoproteins RNA Processing, Post-Transcriptional RNA, Heterogeneous Nuclear/metabolism Ribonucleoproteins/metabolism Ribonucleoproteins, Small Nuclear/metabolism
Chemicals
Heterogeneous-Nuclear Ribonucleoproteins RNA, Heterogeneous Nuclear Ribonucleoproteins Ribonucleoproteins, Small Nuclear
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Matunis E L
Howard Hughes Medical Institute, University of Pennsylvania School of Medicine, Philadelphia 19104-6148.
Matunis M J
Dreyfuss G
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.121.2.219
Published
1993-04-00
Pages
219-28
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2200106
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.121.2.219 PMC Full Text View journal details
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