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PMID: 3181133 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Two proteins crosslinked to RNA containing the adenovirus L3 poly(A) site require the AAUAAA sequence for binding.

The EMBO journal ·Vol. 7 ·No. 10 ·1988-10-00 ·Pages 3159-69

Moore CL, Chen J, Whoriskey J

Abstract

The major proteins crosslinked by UV light to RNA containing the adenovirus-2 L3 poly(A) site are species of 155, 68 and 38 kd mol. wt (p155, p68 and p38). Mutation of AAUAAA to AAGAAA prevented cross-linking of the two larger proteins and destroyed the ability of the RNA to compete for binding of these proteins. However, association of p155 and p68 with precursor was unaffected by deletion of sequences downstream of the poly(A) site critical for in vitro polyadenylation. These two proteins are in the polyadenylation-specific, but not the nonspecific complexes detected by electrophoresis in nondenaturing gels. In addition, p155 and p68 are not found on RNA which has been processed. p155 bound a 15-nt oligomer containing AAUAAA, and thus does not require extended RNA sequence for interaction with RNA. Identified by immunoprecipitation with specific antibody, p38 is the C protein of heterogeneous ribonucleoprotein particles (hmRNPs). While p155 has an Sm epitope, it is not associated with snRNPs containing trimethylated guanosine caps.

MeSH Terms
Adenosine Triphosphate/metabolism Adenoviruses, Human/genetics Autoantibodies/immunology Cell-Free System Cross-Linking Reagents HeLa Cells Kinetics Molecular Weight Nuclear Proteins/metabolism Nucleic Acid Precursors/metabolism Poly A/genetics Precipitin Tests RNA Processing, Post-Transcriptional RNA, Messenger/metabolism Regulatory Sequences, Nucleic Acid Ribonucleoproteins/immunology,metabolism
Chemicals
Autoantibodies Cross-Linking Reagents Nuclear Proteins Nucleic Acid Precursors RNA, Messenger Ribonucleoproteins Poly A Adenosine Triphosphate
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Moore C L
Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, MA 02111.
Chen J
Whoriskey J
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1988-10-00
Pages
3159-69
Language
English
Region
England
NLM ID
8208664
PMCID
PMC454706
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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