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PMID: 3627995 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of nuclear cap specific proteins in HeLa cells.

Nucleic acids research ·Vol. 15 ·No. 16 ·1987-08-25 ·Pages 6489-500

Rozen F, Sonenberg N

Abstract

Two polypeptides of apparent molecular mass of 20 and 115 kilodaltons in nuclear fractions from HeLa cells were shown to recognize and be crosslinked to the cap structure of eukaryotic mRNAs in a cap-dependent fashion. Crosslinking of the 20 and 115 kDa polypeptides was sensitive to inhibition by low concentrations of the cap analogue m7GDP and resistant to inhibition by high KCl concentrations. In addition, crosslinking of these polypeptides to the cap structure occurred in nuclear extracts prepared from poliovirus-infected cells, under conditions where cytoplasmic cap binding proteins were incapable of interacting with the mRNA cap structure. The possible function of nuclear cap binding proteins is discussed.

MeSH Terms
Carrier Proteins/isolation & purification,metabolism,radiation effects Cell Nucleus/analysis HeLa Cells/analysis Humans Molecular Weight Neoplasm Proteins/isolation & purification,metabolism Photochemistry Potassium Chloride/pharmacology RNA Cap-Binding Proteins RNA Caps/metabolism,radiation effects Ultraviolet Rays
Chemicals
Carrier Proteins Neoplasm Proteins RNA Cap-Binding Proteins RNA Caps Potassium Chloride
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Rozen F
Sonenberg N
References (21)
21 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.16.6489
Published
1987-08-25
Pages
6489-500
Language
English
Region
England
NLM ID
0411011
PMCID
PMC306118
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/15.16.6489 PMC Full Text View journal details
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