Home LiteratureArticle Details
PMID: 217002 Published · ppublish English Journal Article

A polypeptide in eukaryotic initiation factors that crosslinks specifically to the 5'-terminal cap in mRNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 10 ·1978-10-00 ·Pages 4843-7

Sonenberg N, Morgan MA, Merrick WC, Shatkin AJ

Abstract

Protein synthesis initiation factors prepared from rabbit reticulocyte and mouse ascites ribosomes were tested for the ability to crosslink to the 5' cap of mRNA. Crosslinking of one polypeptide of apparent molecular weight 24,000 was inhibited by the cap analogs, m7GMP and m7GDP, indicating a specific interaction with the cap. Although specific crosslinking of the 24,000 molecular weight polypeptide was found with eukaryotic initiation factor 3 and to a lesser extent with initiation factor 4B, both of these factors contained less than stoichiometric amounts of this polypeptide. The crosslinking method provides a highly sensitive and specific assay for cap-binding proteins and should facilitate their purification for functional studies.

MeSH Terms
Chromatography, DEAE-Cellulose Guanosine Diphosphate/analogs & derivatives Guanosine Monophosphate/analogs & derivatives Guanosine Triphosphate/analogs & derivatives,metabolism Oligonucleotides/metabolism Oligoribonucleotides/metabolism Peptide Initiation Factors/metabolism Protein Binding RNA, Messenger/metabolism
Chemicals
Oligonucleotides Oligoribonucleotides Peptide Initiation Factors RNA, Messenger Guanosine Diphosphate Guanosine Monophosphate Guanosine Triphosphate
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Sonenberg N
Morgan M A
Merrick W C
Shatkin A J
References (32)
32 references, click to expand
  1. Effect of hemin on site-specific phosphorylation of eukaryotic initiation factor 2.
    Proc Natl Acad Sci U S A. 1978 Feb;75(2):789-93 PMID: 273242
  2. Translational control: recognition of the methylated 5' end and an internal sequence in eukaryotic mRNA by the initiation factor that binds methionyl-tRNAfMet.
    Proc Natl Acad Sci U S A. 1978 Feb;75(2):650-4 PMID: 273228
  3. Importance of 5'-terminal blocking structure to stabilize mRNA in eukaryotic protein synthesis.
    Proc Natl Acad Sci U S A. 1977 Jul;74(7):2734-8 PMID: 197518
  4. RNA-binding proteins of rabbit reticulocytes contain the two elongation factors and some of the initiation factors of translation.
    FEBS Lett. 1978 Apr 1;88(1):21-6 PMID: 639987
  5. Purification and characterization of initiation factors IF-E4 and IF-E6 from rabbit reticulocytes.
    J Biol Chem. 1977 Aug 25;252(16):5798-803 PMID: 885883
  6. International symposium on protein synthesis. Summary of Fogarty Center-NIH Workshop held in Bethesda, Maryland on 18-20 October, 1976.
    FEBS Lett. 1977 Apr 1;76(1):1-10 PMID: 323054
  7. 5'-Terminal structure and mRNA stability.
    Nature. 1977 Mar 17;266(5599):235-9 PMID: 557727
  8. Purification and properties of rabbit reticulocyte protein synthesis initiation factors M2Balpha and M2Bbeta.
    J Biol Chem. 1976 Sep 25;251(18):5551-7 PMID: 965377
  9. Differential synthesis of blocked and unblocked 5'-termini in reovirus mRNA: effect of pyrophosphate and pyrophosphatase.
    Proc Natl Acad Sci U S A. 1976 Oct;73(10):3448-52 PMID: 185613
  10. Absence of 5' terminal capping in encephalomyocarditis virus RNA.
    Nucleic Acids Res. 1976 Oct;3(10):2771-87 PMID: 186763
  11. Reovirus mRNA can be covalently crosslinked via the 5' cap to proteins in initiation complexes.
    Proc Natl Acad Sci U S A. 1977 Oct;74(10):4288-92 PMID: 270673
  12. The mechanism of action of protein synthesis initiation factors from rabbit reticulocytes.
    J Biol Chem. 1978 May 10;253(9):3078-87 PMID: 641056
  13. Initiation of mammalian protein synthesis. II. The assembly of the initiation complex with purified initiation factors.
    J Mol Biol. 1977 Nov;116(4):755-67 PMID: 592399
  14. Initiation of mammalian protein synthesis. I. Purification and characterization of seven initiation factors.
    J Mol Biol. 1977 Nov;116(4):727-53 PMID: 592398
  15. Removal of 5'-terminal m7G from eukaryotic mRNAs by potato nucleotide pyrophosphatase and its effect on translation.
    Nucleic Acids Res. 1977 Sep;4(9):3065-81 PMID: 909799
  16. Characterization of GTP-dependent Met-tRNAf binding protein.
    J Biol Chem. 1977 Jun 10;252(11):3843-7 PMID: 863906
  17. Comparison of mRNA binding by Met-tRNAf binding protein and mRNA-associated proteins.
    J Biol Chem. 1977 Jan 10;252(1):392-8 PMID: 833127
  18. Purification and characterization of initiation factor IF-E3 from rabbit reticulocytes.
    Proc Natl Acad Sci U S A. 1976 Sep;73(9):3005-9 PMID: 1067598
  19. Capping of eucaryotic mRNAs.
    Cell. 1976 Dec;9(4 PT 2):645-53 PMID: 1017010
  20. Influence of 5'-terminal m7G and 2'--O-methylated residues on messenger ribonucleic acid binding to ribosomes.
    Biochemistry. 1976 Dec 28;15(26):5761-8 PMID: 1009086
  21. Initiation of mammalian protein synthesis: the importance of ribosome and initiation factor quality for the efficiency of in vitro systems.
    J Mol Biol. 1973 Feb 19;73(3):329-49 PMID: 4686199
  22. Purification and characterization of two initiation factors required for maximal activity of a highly fractionated globin mRNA translation system.
    Proc Natl Acad Sci U S A. 1976 Aug;73(8):2584-8 PMID: 1066667
  23. Translational discrimination of 'capped' and 'non-capped' mRNAS: inhibition of a series of chemical analogs of m7GpppX.
    FEBS Lett. 1976 May 1;64(2):326-31 PMID: 179883
  24. Effect of 5'-terminal structure and base composition on polyribonucleotide binding to ribosomes.
    J Mol Biol. 1976 Jul 5;104(3):637-58 PMID: 950671
  25. Purification and characterization of homogeneous initiation factor M2A from rabbit reticulocytes.
    J Biol Chem. 1975 Jul 25;250(14):5556-62 PMID: 1095581
  26. Preparation of rabbit reticulocyte initiation factor IF-M3.
    Methods Enzymol. 1974;30:136-41 PMID: 4853751
  27. A protein binding the methylated 5'-terminal sequence, m7GpppN, of eukaryotic messenger RNA.
    Proc Natl Acad Sci U S A. 1976 May;73(5):1559-63 PMID: 1064023
  28. Interaction of poly(A) and mRNA with eukaryotic initiator met-tRNA-f binding factor: identification of this activity on reticulocyte ribonucleic acid protein particles.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):1021-5 PMID: 1055361
  29. Inhibition of initiation of protein synthesis by 7-methylguanosine-5'-monophosphate.
    Proc Natl Acad Sci U S A. 1976 Jan;73(1):19-23 PMID: 1061116
  30. Inhibition of the transition of a 40 S ribosome-Met-tRNA-i-Met complex to an 80 S ribosome-Met-tRNA-i-Met- complex by 7-Methylguanosine-5'-phosphate.
    Nature. 1976 Mar 25;260(5549):359-60 PMID: 1256580
  31. Evidence for role of m7G5'-phosphate group in recognition of eukaryotic mRNA by initiation factor IF-M3.
    Nature. 1976 May 27;261(5558):291-4 PMID: 944862
  32. Purification and physical properties of homogeneous initiation factor MP from rabbit reticulocytes.
    J Biol Chem. 1975 Dec 10;250(23):9067-75 PMID: 1194277
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.75.10.4843
Published
1978-10-00
Pages
4843-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC336217
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.75.10.4843 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2