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PMID: 6954488 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Inactivation of cap-binding proteins accompanies the shut-off of host protein synthesis by poliovirus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 11 ·1982-06-00 ·Pages 3447-51

Lee KA, Sonenberg N

Abstract

Infection of HeLa cells with poliovirus results in a rapid shut-off of host protein synthesis. It has been suggested that inactivation of a protein that binds to the cap structure of cellular mRNAs would explain the selective inhibition of host protein synthesis because the naturally uncapped poliovirus RNA can be translated by a cap-independent mechanism. To test directly for the presence of cap-binding proteins in poliovirus-infected and mock-infected cells, we analyzed initiation factor preparations for their ability to specifically crosslink to the 5' cap structure of oxidized reovirus mRNA. The data presented here show that the crosslinking ability of the different cap-binding proteins (24-, 28-, 32-, 50-, and 80-kilodalton polypeptides) is reduced in preparations from poliovirus-infected as compared to mock-infected cells. This reduction correlates with the inability of initiation factor preparations from infected cells to restore translation of capped mRNAs in extracts of poliovirus-infected cells. In addition, initiation factor preparations from poliovirus-infected cells have the ability to rapidly inactivate cap-binding proteins and can also impair the restoring activity of initiation factors from mock-infected cells.

MeSH Terms
Carrier Proteins/antagonists & inhibitors Cell-Free System Gene Expression Regulation HeLa Cells Peptide Initiation Factors/metabolism Poliomyelitis/metabolism Protein Biosynthesis RNA Cap-Binding Proteins
Chemicals
Carrier Proteins Peptide Initiation Factors RNA Cap-Binding Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lee K A
Sonenberg N
References (31)
31 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.11.3447
Published
1982-06-00
Pages
3447-51
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346437
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.79.11.3447 PMC Full Text View journal details
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