Home LiteratureArticle Details
PMID: 6889136 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

CAP binding proteins associated with the nucleus.

Nucleic acids research ·Vol. 11 ·No. 17 ·1983-09-10 ·Pages 5821-35

Patzelt E, Blaas D, Kuechler E

Abstract

Cap binding proteins of HeLa cells were identified by photo-affinity labelling using the cap analogue gamma-[32P]-[4-(benzoyl-phenyl)methylamido]-7-methylguanosine-5'- triphosphate. Photoreaction with whole cell homogenates resulted in specific labelling of five major polypeptides. The small molecular weight polypeptide appeared to be identical to the 24 000 to 26 000 dalton cap binding protein previously identified in initiation factors. A cap binding protein of 37 000 dalton was found in initiation factors as well as in preparations of crude nuclei. It was released from nuclei by washing with buffer of moderate salt concentration. Three high molecular weight cap binding proteins (approximately 120 000, approximately 89 000, approximately 80 000 dalton) were found in the nuclear fraction and were only partly released upon nuclease digestion and high salt extraction.

MeSH Terms
Affinity Labels Antibodies, Monoclonal Carrier Proteins/isolation & purification Cell Nucleus/analysis Guanosine Triphosphate/analogs & derivatives HeLa Cells/analysis Humans Macromolecular Substances Molecular Weight RNA Cap-Binding Proteins RNA Caps/isolation & purification
Chemicals
Affinity Labels Antibodies, Monoclonal Carrier Proteins Macromolecular Substances RNA Cap-Binding Proteins RNA Caps gamma-(4-(benzoylphenyl)methylamido)-7-methylguanosine-5'-triphosphate Guanosine Triphosphate
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Patzelt E
Blaas D
Kuechler E
References (37)
37 references, click to expand
  1. Translation of poliovirus RNA in vitro: changes in cleavage pattern and initiation sites by ribosomal salt wash.
    Virology. 1979 Sep;97(2):396-405 PMID: 224589
  2. Eukaryotic mRNA cap binding protein: purification by affinity chromatography on sepharose-coupled m7GDP.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4345-9 PMID: 291969
  3. Isolation and characterization of the nuclear matrix in Friend erythroleukemia cells: chromatin and hnRNA interactions with the nuclear matrix.
    Cell. 1979 Dec;18(4):1079-90 PMID: 293220
  4. hnRNA and its attachment to a nuclear protein matrix.
    J Cell Biol. 1981 Mar;88(3):554-63 PMID: 7217204
  5. A unique cap(m7GpppXm)-dependent influenza virion endonuclease cleaves capped RNAs to generate the primers that initiate viral RNA transcription.
    Cell. 1981 Mar;23(3):847-58 PMID: 6261960
  6. ATP/Mg++-dependent cross-linking of cap binding proteins to the 5' end of eukaryotic mRNA.
    Nucleic Acids Res. 1981 Apr 10;9(7):1643-56 PMID: 7232223
  7. Presence of the cap-binding protein in initiation factor preparations from poliovirus-infected HeLa cells.
    J Virol. 1981 May;38(2):438-45 PMID: 6264121
  8. RNA is synthesized at the nuclear cage.
    Nature. 1981 Aug 6;292(5823):552-5 PMID: 6166873
  9. Two forms of purified m7G-cap binding protein with different effects on capped mRNA translation in extracts of uninfected and poliovirus-infected HeLa cells.
    J Biol Chem. 1981 Aug 10;256(15):7691-4 PMID: 6267020
  10. The nuclear matrix of duck erythroblasts is associated with globin mRNA coding sequences but not with the major proteins of 40S nuclear RNP.
    Exp Cell Res. 1981 Dec;136(2):435-45 PMID: 7308318
  11. Role of two of the influenza virus core P proteins in recognizing cap 1 structures (m7GpppNm) on RNAs and in initiating viral RNA transcription.
    Proc Natl Acad Sci U S A. 1981 Dec;78(12):7355-9 PMID: 6950380
  12. Cap-recognizing protein of influenza virus.
    Virology. 1982 Jan 15;116(1):339-48 PMID: 6801849
  13. Characterization of eukaryotic initiation factor 4A, a protein involved in ATP-dependent binding of globin mRNA.
    J Biol Chem. 1982 May 10;257(9):5246-52 PMID: 7068683
  14. Association of globin ribonucleic acid and its precursors with the chicken erythroblast nuclear matrix.
    Biochemistry. 1982 Feb 16;21(4):764-71 PMID: 6176264
  15. Adenoviral heterogeneous nuclear RNA is associated with the host nuclear matrix during splicing.
    J Mol Biol. 1982 Jan 5;154(1):103-19 PMID: 6176712
  16. Non-histone proteins and long-range organization of HeLa interphase DNA.
    J Mol Biol. 1982 Apr 5;156(2):325-44 PMID: 6896344
  17. Association of cap-binding protein with eucaryotic initiation factor 3 in initiation factor preparations from uninfected and poliovirus-infected HeLa cells.
    J Virol. 1982 Apr;42(1):200-7 PMID: 6283140
  18. Association of a Mr 50,000 cap-binding protein with the cytoskeleton in baby hamster kidney cells.
    Proc Natl Acad Sci U S A. 1982 May;79(9):2927-31 PMID: 7045875
  19. Inactivation of cap-binding proteins accompanies the shut-off of host protein synthesis by poliovirus.
    Proc Natl Acad Sci U S A. 1982 Jun;79(11):3447-51 PMID: 6954488
  20. Identification of the cap binding protein of influenza virus.
    Nucleic Acids Res. 1982 Aug 11;10(15):4803-12 PMID: 7133998
  21. Ribonucleic acid precursors are associated with the chick oviduct nuclear matrix.
    Biochemistry. 1982 Sep 28;21(20):4945-53 PMID: 6182907
  22. Probing the function of the eukaryotic 5'-cap structure using monoclonal antibodies to cap-binding proteins.
    Curr Top Cell Regul. 1982;21:65-88 PMID: 6754270
  23. On the localization and transport of specific adenoviral mRNA-sequences in the late infected HeLa cell.
    Nucleic Acids Res. 1982 Oct 11;10(19):6131-45 PMID: 6128726
  24. Inhibition of HeLa cell protein synthesis following poliovirus infection correlates with the proteolysis of a 220,000-dalton polypeptide associated with eucaryotic initiation factor 3 and a cap binding protein complex.
    J Biol Chem. 1982 Dec 25;257(24):14806-10 PMID: 6294080
  25. The nuclear matrix: three-dimensional architecture and protein composition.
    Cell. 1982 Jul;29(3):847-58 PMID: 7151171
  26. Sequence dependent interaction of hnRNP proteins with late adenoviral transcripts.
    Nucleic Acids Res. 1982 Nov 25;10(22):7115-31 PMID: 6296766
  27. Role of mRNA competition in regulating translation: further characterization of mRNA discriminatory initiation factors.
    Proc Natl Acad Sci U S A. 1983 Feb;80(3):663-7 PMID: 6572361
  28. SV40 virions and viral RNA metabolism are associated with cellular substructures.
    EMBO J. 1982;1(10):1225-31 PMID: 6327258
  29. Probing the function of the eucaryotic 5' cap structure by using a monoclonal antibody directed against cap-binding proteins.
    Cell. 1981 Dec;27(3 Pt 2):563-72 PMID: 6101207
  30. Localization of cap-binding protein in subcellular fractions of HeLa cells.
    Mol Cell Biol. 1982 Dec;2(12):1639-43 PMID: 14582205
  31. RNA metabolism in the HeLa cell nucleus.
    J Mol Biol. 1966 May;17(1):117-30 PMID: 5961144
  32. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  33. Initiation of mammalian protein synthesis: the importance of ribosome and initiation factor quality for the efficiency of in vitro systems.
    J Mol Biol. 1973 Feb 19;73(3):329-49 PMID: 4686199
  34. Aromatic ketone derivatives of aminoacyl-tRNA as photoaffinity labels for ribosomes.
    Methods Enzymol. 1977;46:676-83 PMID: 242736
  35. Heterogeneous nuclear RNA-protein fibers in chromatin-depleted nuclei.
    J Cell Biol. 1978 Sep;78(3):663-74 PMID: 701354
  36. A polypeptide in eukaryotic initiation factors that crosslinks specifically to the 5'-terminal cap in mRNA.
    Proc Natl Acad Sci U S A. 1978 Oct;75(10):4843-7 PMID: 217002
  37. Isolation and characterization of an RNA-containing nuclear matrix from Tetrahymena macronuclei.
    Biochemistry. 1979 May 1;18(9):1782-8 PMID: 107967
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.17.5821
Published
1983-09-10
Pages
5821-35
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326320
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/11.17.5821 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2