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PMID: 7045875 Published · ppublish English Journal Article

Association of a Mr 50,000 cap-binding protein with the cytoskeleton in baby hamster kidney cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 9 ·1982-05-00 ·Pages 2927-31

Zumbé A, Stähli C, Trachsel H

Abstract

A monoclonal antibody directed against eukaryotic mRNA 5'-cap-binding protein (anti-CBP antibody) was used to localize cap-binding protein (CBP) in BHK-21 baby hamster kidney cells by immunofluorescence microscopy. It was found that the antibody reacts with a fibrous network extending through the cytoplasm in a radial arrangement. The network behaves like intermediate filaments in colchicine-treated cells, suggesting a direct or indirect linkage of CBP with intermediate filaments. The association of CBP with a cytoskeletal element was further confirmed by isolation of proteins from Triton X-100-extracted cells and identification of CBP in the cytoskeletal fraction with anti-CBP antibody. The major polypeptide reacting with anti-CBP antibody is a Mr 50,000 component. Tryptic peptide mapping showed that this polypeptide is related to a Mr 24,000 polypeptide identified as CBP in earlier experiments [Sonenberg, N., Morgan, M. A., Testa, D., Colonna, R. J. & Shatkin, A. J. (1978) Proc. Natl. Acad. Sci. USA 75, 4843-4847].

MeSH Terms
Animals Antibodies, Monoclonal Carrier Proteins/immunology,metabolism Cell Line Cricetinae Cytoskeleton/metabolism Fluorescent Antibody Technique Microtubules/metabolism Molecular Weight Peptide Fragments/analysis Peptide Initiation Factors/metabolism RNA Cap-Binding Proteins RNA, Messenger/metabolism
Chemicals
Antibodies, Monoclonal Carrier Proteins Peptide Fragments Peptide Initiation Factors RNA Cap-Binding Proteins RNA, Messenger
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Zumbé A
Stähli C
Trachsel H
References (33)
33 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.9.2927
Published
1982-05-00
Pages
2927-31
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346321
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.79.9.2927 PMC Full Text View journal details
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