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PMID: 493138 Published · ppublish English Journal Article

Interaction of a limited set of proteins with different mRNAs and protection of 5'-caps against pyrophosphatase digestion in initiation complexes.

Nucleic acids research ·Vol. 7 ·No. 1 ·1979-09-11 ·Pages 15-29

Sonenberg N, Morgan MA, Testa D, Colonno RJ, Shatkin AJ

Abstract

A variety of 5'-3H-methyl-labeled, oxidized viral mRNAs were used as probes for detecting in wheat germ initiation complexes proteins that interact with, and can be cross-linked to, the 5'-cap structure. A limited and reproducible set of specific proteins was obtained with the different mRNAs. The binding of these proteins to the 5'-end of mRNA apparently results in protection against nucleotide pyrophosphatase digestion of the cap even in initiation complexes in which the 5'-end is susceptible to pancreatic RNase digestion. Cross-linked proteins from mammalian initiation complexes comigrated with several of the subunits of similarly treated eIF-3. A model for cap binding protein interaction with mRNA cap during initiation of translation is suggested.

MeSH Terms
Animals Macromolecular Substances Molecular Weight Peptide Chain Initiation, Translational Peptide Initiation Factors/metabolism Plants/metabolism RNA Caps/metabolism RNA, Messenger/metabolism Rabbits Reoviridae Reticulocytes/metabolism Ribosomal Proteins/metabolism Triticum/metabolism Vaccinia virus
Chemicals
Macromolecular Substances Peptide Initiation Factors RNA Caps RNA, Messenger Ribosomal Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Sonenberg N
Morgan M A
Testa D
Colonno R J
Shatkin A J
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36 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/7.1.15
Published
1979-09-11
Pages
15-29
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327993
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/7.1.15 PMC Full Text View journal details
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