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PMID: 329284 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Isolation and preliminary characterization of 10-nm filaments from baby hamster kidney (BHK-21) cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 6 ·1977-06-00 ·Pages 2422-6

Starger JM, Goldman RD

Abstract

A procedure for isolating 10-nm filaments as juxtanuclear caps from normal and colchicine-treated BHK-21 cells is described. These aggregates of 10-nm filaments retain their birefringence and their structural integrity. The major proteins comprising the filament preparations can be resolved as two bands on sodium dodecyl sulfate gels, corresponding to approximate molecular weights of 54,000 and 55,000.

MeSH Terms
Cell Line Colchicine/pharmacology Kidney/drug effects,ultrastructure Microscopy, Electron Microscopy, Phase-Contrast Organoids
Chemicals
Colchicine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Starger J M
Goldman R D
References (28)
28 references, click to expand
  1. A MICRO-BIURET METHOD FOR ESTIMATING PROTEINS.
    Anal Biochem. 1964 Dec;9:401-10 PMID: 14239476
  2. Fine structure of desmosomes. , hemidesmosomes, and an adepidermal globular layer in developing newt epidermis.
    J Cell Biol. 1966 Jan;28(1):51-72 PMID: 5901500
  3. Immunological characterization of the subunit of the 100 A filaments from muscle cells.
    Proc Natl Acad Sci U S A. 1976 Dec;73(12):4344-8 PMID: 1069986
  4. A filamentous cytoskeleton in vertebrate smooth muscle fibers.
    J Cell Biol. 1976 Mar;68(3):539-56 PMID: 1025153
  5. Fibrillar systems in cell motility.
    Ciba Found Symp. 1973;14:83-107 PMID: 4130536
  6. Potassium chloride-insoluble myofilaments in vertebrate smooth muscle cells.
    Exp Cell Res. 1971 Jun;66(2):417-25 PMID: 4104114
  7. Actin and myosin and cell movement.
    CRC Crit Rev Biochem. 1974 Jan;2(1):1-65 PMID: 4273099
  8. Microtubule assembly in the absence of added nucleotides.
    Proc Natl Acad Sci U S A. 1973 Mar;70(3):765-8 PMID: 4514990
  9. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.
    Biochemistry. 1971 Jun 22;10(13):2606-17 PMID: 4326772
  10. The use of heavy meromyosin binding as an ultrastructural cytochemical method for localizing and determining the possible functions of actin-like microfilaments in nonmuscle cells.
    J Histochem Cytochem. 1975 Jul;23(7):529-42 PMID: 1095652
  11. Biochemistry of the filaments of brain.
    Proc Natl Acad Sci U S A. 1976 Feb;73(2):529-33 PMID: 54920
  12. Different distribution pattern of 100-A filaments in resting and locomotive leukaemia cells.
    Nature. 1976 Jun 17;261(5561):604-6 PMID: 180418
  13. Neurofilament disguise, destruction and discipline.
    Nature. 1975 Aug 14;256(5518):586-9 PMID: 170526
  14. On the relationship of brain filaments to microtubules.
    J Neurochem. 1974 Mar;22(3):321-6 PMID: 4829957
  15. The protein subunit of calf brain neurofilament.
    J Neurobiol. 1974;5(2):119-33 PMID: 4835398
  16. The role of three cytoplasmic fibers in BHK-21 cell motility. I. Microtubules and the effects of colchicine.
    J Cell Biol. 1971 Dec;51(3):752-62 PMID: 4942774
  17. Birefringent filamentous organelle in BHK-21 cells and its possible role in cell spreading and motility.
    Science. 1970 Jul 17;169(3942):286-8 PMID: 4915821
  18. The distribution of actin in non-muscle cells. The use of actin antibody in the localization of actin within the microfilament bundles of mouse 3T3 cells.
    Exp Cell Res. 1975 Feb;90(2):333-44 PMID: 1089542
  19. Rings of intermediate (100 A) filament bundles in the perinuclear region of vascular endothelial cells. Their mobilization by colcemid and mitosis.
    J Cell Biol. 1976 Aug;70(2 pt 1):459-66 PMID: 945806
  20. Heterogeneity of the alpha subunit of tubulin and the variability of tubulin within a single organism.
    J Cell Biol. 1976 May;69(2):301-12 PMID: 1262392
  21. The occurrence and possible role of 80-100 A filaments in PtKl cells.
    Exp Cell Res. 1975 Dec;96(2):303-10 PMID: 1193180
  22. Isolation of filaments from brain.
    Science. 1971 Dec 17;174(4015):1242-5 PMID: 5133446
  23. Fibrillar proteins from squid axons. I. Neurofilament protein.
    J Mol Biol. 1970 Sep 28;52(3):415-28 PMID: 5492289
  24. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  25. Maternal characteristics of hatching enzymes in hybrid sea urchin embryos.
    Exp Cell Res. 1969 Oct;57(2):159-66 PMID: 5388416
  26. Effects of mitotic spindle inhibitors on neurotubules and neurofilaments in anterior horn cells.
    J Cell Biol. 1968 Jul;38(1):224-9 PMID: 5691976
  27. Mitosis and intermediate-sized filaments in developing skeletal muscle.
    J Cell Biol. 1968 Sep;38(3):538-55 PMID: 5664223
  28. Localization of the neurofilament protein in neuroblastoma cells by immunofluorescent staining.
    Proc Natl Acad Sci U S A. 1976 Sep;73(9):3192-6 PMID: 787987
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.6.2422
Published
1977-06-00
Pages
2422-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432184
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.74.6.2422 PMC Full Text View journal details
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