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PMID: 3479794 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A poly(A) addition site and a downstream termination region are required for efficient cessation of transcription by RNA polymerase II in the mouse beta maj-globin gene.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 23 ·1987-12-00 ·Pages 8306-10

Logan J, Falck-Pedersen E, Darnell JE, Shenk T

Abstract

Sequence elements within the mouse beta maj-globin transcription unit required for efficient termination of transcription by RNA polymerase II have been delineated. To facilitate nascent-chain analysis of termination, the DNA segment in which transcription ceases was introduced into the adenovirus chromosome within its E1A transcription unit. Two beta-globin DNA elements were required to effect efficient termination: an upstream sequence that includes two poly(A) addition signals and a downstream region previously shown to be where RNA synthesis stops. The role of poly(A) addition in termination was established by introduction of several single base pair substitutions into the AATAAA polyadenylylation motifs. These mutations inhibited both polyadenylylation and termination within the beta-globin DNA segment. Therefore, poly(A) addition appears to be a prerequisite for efficient termination.

MeSH Terms
Animals DNA Mutational Analysis Genes, Regulator Globins/genetics Mice Poly A/genetics RNA Polymerase II/genetics RNA, Messenger/genetics Terminator Regions, Genetic Transcription, Genetic
Chemicals
RNA, Messenger Poly A Globins RNA Polymerase II
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Logan J
Agricultural Research Division, American Cyanamid Company, Princeton, NJ 08540.
Falck-Pedersen E
Darnell J E
Shenk T
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.23.8306
Published
1987-12-00
Pages
8306-10
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC299531
Subset
IM
Grants
NCI NIH HHS · CA-38965 · United States
External Links
PubMed source DOI: 10.1073/pnas.84.23.8306 PMC Full Text View journal details
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