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PMID: 6095210 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Transcription termination occurs within a 1000 base pair region downstream from the poly(A) site of the mouse beta-globin (major) gene.

Nucleic acids research ·Vol. 12 ·No. 22 ·1984-11-26 ·Pages 8723-31

Citron B, Falck-Pedersen E, Salditt-Georgieff M, Darnell JE

Abstract

Transcription of RNA from the beta-globin (major) gene in nuclei from induced mouse erythroleukemia cells terminates within the region between 700 to 2000 bases downstream from the poly(A) addition site, but not at particularly favored sites. In addition we present the first analysis of in vivo labeled RNA from a cell transcription unit that shows RNA termination in the same region as diagnosed by analysis of in vitro labeled RNA. The region in which termination occurs is contained in 1414 bp that were sequenced beginning 600 bases downstream from the poly(A) site. There is an increased frequency of the sequence AATAAA at the beginning and a stem and loop structure followed by a string of Ts near the end of this region.

MeSH Terms
Animals Base Composition Base Sequence Cell Line DNA Restriction Enzymes Genes Globins/genetics Leukemia, Experimental/metabolism Mice Nucleic Acid Hybridization Poly A/analysis Transcription, Genetic
Chemicals
Poly A Globins DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Citron B
Falck-Pedersen E
Salditt-Georgieff M
Darnell J E
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.22.8723
Published
1984-11-26
Pages
8723-31
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320410
Subset
IM
Grants
PHS HHS · A107233-08 · United States
NCI NIH HHS · CA 16006-11 · United States
NCI NIH HHS · CA 18213-09 · United States
Databases
GENBANK
J00413, K01748, K03545, X00624
External Links
PubMed source DOI: 10.1093/nar/12.22.8723 PMC Full Text View journal details
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