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PMID: 6157696 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Addition of poly(A) to nuclear RNA occurs soon after RNA synthesis.

The Journal of cell biology ·Vol. 86 ·No. 3 ·1980-09-00 ·Pages 844-8

Salditt-Georgieff M, Harpold M, Sawicki S, Nevins J, Darnell JE

Abstract

A kinetic analysis of the appearance of [3H]uridine label in RNA sequences that neighbor poly(A), as well as the incorporation of [3H]adenosine label into both the RNA chain and the poly(A) of poly(A)-containing molecules, shows that poly(A) is added within a minute or so after RNA chain synthesis in Chinese hamster ovary cells and HeLa cells. Previous conclusions by several groups (5-7) that poly(A) might be added as long as 20-30 min after RNA synthesis appear to be in error, and the present conclusion seems much more in line with several different types of recent studies with specific mRNAs that suggest prompt poly(A) addition (13-16).

MeSH Terms
Animals Cell Line Cell Nucleus/metabolism Cricetinae Cytoplasm/metabolism Dactinomycin/pharmacology Humans Kinetics Nucleic Acid Precursors/metabolism Poly A/metabolism RNA, Heterogeneous Nuclear/metabolism RNA, Messenger/biosynthesis
Chemicals
Nucleic Acid Precursors RNA, Heterogeneous Nuclear RNA, Messenger Dactinomycin Poly A
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Salditt-Georgieff M
Harpold M
Sawicki S
Nevins J
Darnell J E
References (31)
31 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.86.3.844
Published
1980-09-00
Pages
844-8
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2110690
Subset
IM
Grants
NCI NIH HHS · CA 18213-04 · United States
NIGMS NIH HHS · GM 26765-01 · United States
External Links
PubMed source DOI: 10.1083/jcb.86.3.844 PMC Full Text View journal details
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