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PMID: 6136617 Published · ppublish English Journal Article

Polyadenylic acid addition sites in the adenovirus type 2 major late transcription unit.

Journal of virology ·Vol. 48 ·No. 1 ·1983-10-00 ·Pages 127-34

Le Moullec JM, Akusjärvi G, Stålhandske P, Pettersson U, Chambraud B, Gilardi P, Nasri M, Perricaudet M

Abstract

The cytoplasmic mRNAs which are transcribed from the major late adenovirus promoter can be arranged into five 3'-coterminal families, L1 to L5. We have defined the polyadenylation sites of the mRNAs that belong to the five families at the nucleotide level. From the results, the following conclusions can be made. (i) The hexanucleotide sequence AAUAAA is present at the 3' end of all late adenovirus type 2 mRNAs and precedes the site of polyadenylation by 12 to 30 nucleotides. (ii) Between one and three A residues are present in the genomic sequence at the polyadenylation site. (iii) A sequence with the composition (T)n (A)p (T)q (n, p, q greater than or equal to 1) is found 4 to 24 nucleotides beyond all the adenovirus-specific polyadenylation sites except the 3'-coterminal family L4. This sequence is also found beyond many cellular polyadenylation sites. (iv) The L1 and L2 polyadenylation sites are very similar in structure. The other polyadenylation sites show no apparent sequence relationship, except for the hexanucleotide sequence.

MeSH Terms
Adenoviruses, Human/genetics,metabolism Base Sequence Cloning, Molecular Poly A/metabolism RNA, Messenger/metabolism RNA, Viral/genetics,metabolism Transcription, Genetic
Chemicals
RNA, Messenger RNA, Viral Poly A
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Le Moullec J M
Akusjärvi G
Stålhandske P
Pettersson U
Chambraud B
Gilardi P
Nasri M
Perricaudet M
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.48.1.127-134.1983
Published
1983-10-00
Pages
127-34
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC255329
Subset
IM
Databases
GENBANK
J01917, J01918, J01919, J01920, J01921, J01922, J01923, J01924, J01925, J01926, J01927, J01928, J01929, J01930, J01931, J01932, J01933, J01934, J01935, J01936, J01937, J01938, J01939, J01940, J01941, J01942, J01943, J01944, J01945, J01946
External Links
PubMed source DOI: 10.1128/JVI.48.1.127-134.1983 PMC Full Text View journal details
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