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PMID: 6985479 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Gene and mRNA for precursor polypeptide VI from adenovirus type 2.

Journal of virology ·Vol. 38 ·No. 2 ·1981-05-00 ·Pages 469-82

Akusjärvi G, Persson H

Abstract

We present a 1,040-base-pair-long sequences of adenoviruses type 2 DNA which encodes the complete gene for precursor polypeptide VI (pVI). pVI consists of 250 amino acids amounting to a molecular weight of 26,990. The proteolytic cleavage maturing pVI to virion polypeptide VI removes 33 amino acids from the amino-terminal end of the polypeptide, thus giving the mature polypeptide VI a molecular weight of 23,400. The UAA stop codon terminating pVI translation is separated by 84 nucleotides from the initiator triplet for the hexon gene. Both polypeptides are encoded by the same translational reading frame, suggesting the evolution of pVI and hexon as separate proteins by the introduction of a termination codon and selection of a new splice acceptor site in an ancestral fused polypeptide chain. The splice site where the common tripartite leader is attached to the pVI mRNA precedes the initiator codon for pVI translation by one nucleotide and forms, together with other late splice acceptor sites, a late adenovirus consensus acceptor site. We also demonstrate that the 3' end of the mRNA's belonging to the L2 3'-cotermination family is located only 31 nucleotides upstream from the splice junction of the pVI mRNA. Furthermore, we show that four novel polypeptides of molecular weights 80,000, 39,000, 36,000, and 10,500 are encoded by region L2.

MeSH Terms
Adenoviruses, Human/genetics Base Sequence Codon DNA, Viral Genes, Viral Molecular Weight Protein Biosynthesis Protein Precursors/genetics RNA, Messenger/genetics RNA, Viral/genetics Viral Proteins/genetics
Chemicals
Codon DNA, Viral Protein Precursors RNA, Messenger RNA, Viral Viral Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Akusjärvi G
Persson H
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43 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.38.2.469-482.1981
Published
1981-05-00
Pages
469-82
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC171178
Subset
IM
Databases
GENBANK
J01917, J01918, J01919, J01920, J01921, J01922, J01923, J01924, J01925, J01926, J01927, J01928, J01929, J01930, J01931, J01932, J01933, J01934, J01935, J01936, J01937, J01938, J01939, J01940, J01941, J01942, J01943, J01944, J01945, J01946
External Links
PubMed source DOI: 10.1128/JVI.38.2.469-482.1981 PMC Full Text View journal details
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