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PMID: 6259616 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The sequence of the 3' non-coding region of the hexon mRNA discloses a novel adenovirus gene.

Nucleic acids research ·Vol. 9 ·No. 1 ·1981-01-10 ·Pages 1-17

Akusjärvi G, Zabielski J, Perricaudet M, Pettersson U

Abstract

We report the sequence of a 1164 nucleotide long DNA segment, located between map positions 59.5 and 62.8 on the adenovirus type 2 genome. The sequence comprises the 701 nucleotides long 3' non-coding region of the hexon mRNA as well as several important processing signals. The sequence revealed unexpectedly that the 3' non-coding region of the hexon mRNA contains a 609 nucleotide long uninterrupted translational reading frame following a potential initiator AUG. A late 14S mRNA, corresponding to the open reading frame, could be identified by S1 nuclease mapping and electronmicroscopy. The mRNA shares a poly(A) addition site with the hexon and pVI mRNAs, and carries a leader sequence which is related, and probably identical, to the tripartite leader, found in late adenovirus mRNAs. The junction between the leader and the body of this novel mRNA is located within the coding part of the hexon gene.

MeSH Terms
Adenoviruses, Human/genetics Base Sequence DNA Restriction Enzymes DNA, Viral/genetics Genes, Viral Genetic Code Microscopy, Electron Nucleic Acid Hybridization RNA, Messenger/genetics
Chemicals
DNA, Viral RNA, Messenger DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Akusjärvi G
Zabielski J
Perricaudet M
Pettersson U
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31 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.1.1
Published
1981-01-10
Pages
1-17
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326664
Subset
IM
Databases
GENBANK
J01917, J01918, J01919, J01920, J01921, J01922, J01923, J01924, J01925, J01926, J01927, J01928, J01929, J01930, J01931, J01932, J01933, J01934, J01935, J01936, J01937, J01938, J01939, J01940, J01941, J01942, J01943, J01944, J01945, J01946
External Links
PubMed source DOI: 10.1093/nar/9.1.1 PMC Full Text View journal details
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