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PMID: 2962858 Published · ppublish English Journal Article

In vitro assembly of U1 snRNPs.

The EMBO journal ·Vol. 6 ·No. 11 ·1987-11-00 ·Pages 3479-85

Hamm J, Kazmaier M, Mattaj IW

Abstract

An efficient system for the in vitro assembly of U1 snRNPs is described. RNA-protein interactions in a series of U1 snRNA mutants assembled both in vivo and in vitro were studied in order to verify the accuracy of the system. Two discrete protein binding sites are defined by immunoprecipitation with antibodies against different protein components of the U1 snRNP and a newly developed protein sequestering assay. The U1 snRNP-specific proteins 70K and A require only the 5'-most stem-loop structure of U1 snRNA for binding, the common U snRNP proteins require the conserved Sm binding site (AUnG). Interactions between these two groups of proteins are detected. These results are combined to derive a model of the U1 snRNP structure. The potential use of the in vitro system in the functional analysis of U1 snRNP proteins is discussed.

MeSH Terms
Animals Chromosome Deletion Female Genes Mutation Nucleic Acid Conformation Oocytes/metabolism Ribonucleoproteins/genetics Ribonucleoproteins, Small Nuclear Templates, Genetic Transcription, Genetic Xenopus laevis
Chemicals
Ribonucleoproteins Ribonucleoproteins, Small Nuclear
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hamm J
European Molecular Biology Laboratory, Heidelberg, FRG.
Kazmaier M
Mattaj I W
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34 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-11-00
Pages
3479-85
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553806
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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