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PMID: 2941274 Published · ppublish English Journal Article

Monospecific antibodies reveal details of U2 snRNP structure and interaction between U1 and U2 snRNPs.

The EMBO journal ·Vol. 5 ·No. 5 ·1986-05-00 ·Pages 997-1002

Mattaj IW, Habets WJ, van Venrooij WJ

Abstract

Monospecific antibodies directed against several U small nuclear ribonucleoprotein (U snRNP) particle proteins were affinity purified from a patient's anti-(U1,U2)RNP serum. These were used to demonstrate that: (i) proteins equivalent to the mammalian U2 snRNP-specific A' and B" proteins are present in Xenopus laevis oocytes; (ii) both proteins A' and B" have the same structural requirements for binding to U2 snRNA; (iii) proteins B, B' and D have the same structural requirement for binding to U2 snRNA; (iv) using very high specific activity RNA probes it is possible to detect a fraction of either U1 or U2 snRNA precipitable by antibodies directed against proteins specific for the other U snRNP, indicating an interaction between U1 and U2 snRNPs. The structural requirements of this interaction were studied for the U2 snRNP. All changes made to U2 snRNA or snRNP structure resulted in loss of the interaction with U1 snRNP.

MeSH Terms
Animals Antibodies, Monoclonal Female Nucleic Acid Conformation Oocytes/metabolism RNA, Small Nuclear/analysis Ribonucleoproteins/analysis,genetics Ribonucleoproteins, Small Nuclear Structure-Activity Relationship Transcription, Genetic Xenopus
Chemicals
Antibodies, Monoclonal RNA, Small Nuclear Ribonucleoproteins Ribonucleoproteins, Small Nuclear
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Mattaj I W
Habets W J
van Venrooij W J
References (34)
34 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1986-05-00
Pages
997-1002
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1166893
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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