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PMID: 1834995 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Polypeptide components of Drosophila small nuclear ribonucleoprotein particles.

Nucleic acids research ·Vol. 19 ·No. 21 ·1991-11-11 ·Pages 5877-82

Paterson T, Beggs JD, Finnegan DJ, Lührmann R

Abstract

In eukaryotes splicing of pre-mRNAs is mediated by the spliceosome, a dynamic complex of small nuclear ribonucleoprotein particles (snRNPs) that associate transiently during spliceosome assembly and the splicing reaction. We have purified snRNPs from nuclear extracts of Drosophila cells by affinity chromatography with an antibody specific for the trimethylguanosine (m3G) cap structure of snRNAs U1-U5. The polypeptide components of Drosophila snRNPs have been characterized and shown to consist of a number of proteins shared by all the snRNPs, and some proteins which appear to be specific to individual snRNP particles. On the basis of their apparent molecular weight and antigenicity many of these common and particle specific Drosophila snRNP proteins are remarkably conserved between Drosophila and human spliceosomes. By probing western blots of the Drosophila snRNP polypeptides with a number of antisera raised against human snRNP proteins, Drosophila polypeptides equivalent to many of the HeLa snRNP-common proteins have been identified, as well as candidates for a number of U1, U2 and U5-specific proteins.

MeSH Terms
Animals Antibodies, Monoclonal Base Sequence Blotting, Western Centrifugation, Density Gradient Chromatography, Affinity Drosophila melanogaster/chemistry HeLa Cells/chemistry Humans Molecular Sequence Data Nucleoproteins/analysis,chemistry RNA Caps/analysis,isolation & purification Ribonucleoproteins/chemistry,isolation & purification Ribonucleoproteins, Small Nuclear
Chemicals
Antibodies, Monoclonal Nucleoproteins RNA Caps Ribonucleoproteins Ribonucleoproteins, Small Nuclear
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Paterson T
Institute of Cell and Molecular Biology, University of Edinburgh.
Beggs J D
Finnegan D J
Lührmann R
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/19.21.5877
Published
1991-11-11
Pages
5877-82
Language
English
Region
England
NLM ID
0411011
PMCID
PMC329041
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/19.21.5877 PMC Full Text View journal details
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