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PMID: 2847033 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The C proteins of heterogeneous nuclear ribonucleoprotein complexes interact with RNA sequences downstream of polyadenylation cleavage sites.

Molecular and cellular biology ·Vol. 8 ·No. 10 ·1988-10-00 ·Pages 4477-83

Wilusz J, Feig DI, Shenk T

Abstract

The heterogeneous nuclear ribonucleoprotein C1 and C2 proteins were preferentially cross-linked by treatment with UV light in nuclear extracts to RNAs containing six different polyadenylation signals. The domain required for the interaction was located downstream of the poly(A) cleavage site, since deletion of this segment from several polyadenylation substrate RNAs greatly reduced cross-linking efficiency. In addition, RNAs containing only downstream sequences were efficiently cross-linked to C proteins, while fully processed, polyadenylated RNAs were not. Analysis of mutated variants of the simian virus 40 late polyadenylation signal showed that uridylate-rich sequences located in the region between 30 and 55 nucleotides downstream of the cleavage site were required for efficient cross-linking of C proteins. This downstream domain of the simian virus 40 late poly(A) addition signal has been shown to influence the efficiency of the polyadenylation reaction. However, there was not a strict correlation between cross-linking of C proteins and the efficiency of polyadenylation.

MeSH Terms
Base Sequence Binding Sites Cross-Linking Reagents Heterogeneous-Nuclear Ribonucleoprotein Group C Heterogeneous-Nuclear Ribonucleoproteins In Vitro Techniques Molecular Sequence Data Phosphoproteins/metabolism Poly A/metabolism RNA, Heterogeneous Nuclear/physiology Regulatory Sequences, Nucleic Acid Ribonucleoproteins/physiology Simian virus 40/genetics
Chemicals
C1 HNRNP Cross-Linking Reagents Heterogeneous-Nuclear Ribonucleoprotein Group C Heterogeneous-Nuclear Ribonucleoproteins Phosphoproteins RNA, Heterogeneous Nuclear Ribonucleoproteins Poly A
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wilusz J
Department of Molecular Biology, Princeton University, New Jersey 08544.
Feig D I
Shenk T
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.10.4477-4483.1988
Published
1988-10-00
Pages
4477-83
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365522
Subset
IM
Grants
NCI NIH HHS · CA-38965 · United States
External Links
PubMed source DOI: 10.1128/mcb.8.10.4477-4483.1988 PMC Full Text View journal details
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