Distribution of RNA polymerase on Drosophila polytene chromosomes as studied by indirect immunofluorescence.

Chromosoma ·Vol. 59 ·No. 2 ·1976-12-16 ·Pages 157-65

Plagens U, Greenleaf AL, Bautz EK

Abstract

Using indirect immunofluorescence visualization techniques we investigated the in situ distribution of RNA polymerase B on Drosophila melanogaster polytene chromosomes. The enzyme was found at many sites distributed throughout the genome in a pattern clearly distinct from that observed for histone H1, but it was especially concentrated in puffs induced by heat shock.

MeSH Terms

Animals Chromosomes/enzymology DNA-Directed RNA Polymerases/analysis Drosophila melanogaster Fluorescent Antibody Technique Histones/analysis Hot Temperature RNA Polymerase II/analysis

Chemicals

Histones RNA Polymerase II DNA-Directed RNA Polymerases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Plagens U

Greenleaf A L

Bautz E K

References (18)

18 references, click to expand

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Article Info

Journal

Chromosoma

Abbr.

Chromosoma

ISSN

0009-5915

DOI

10.1007/BF00328484

Published

1976-12-16

Pages

157-65

Language

English

Region

Austria

NLM ID

2985138R

Subset

External Links

PubMed source DOI: 10.1007/BF00328484 View journal details

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Distribution of RNA polymerase on Drosophila polytene chromosomes as studied by indirect immunofluorescence.

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