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PMID: 8404849 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Cognate gene clusters govern invasion of host epithelial cells by Salmonella typhimurium and Shigella flexneri.

The EMBO journal ·Vol. 12 ·No. 10 ·1993-10-00 ·Pages 3779-87

Groisman EA, Ochman H

Abstract

The enteric pathogens Salmonella typhimurium and Shigella flexneri differ in most virulence attributes including infectivity, pathology and host range. We have identified a new assemblage of genes responsible for invasion properties of Salmonella which is remarkably similar in order, arrangement and sequence to the gene cluster controlling the presentation of surface antigens (spa) on the virulence plasmid of Shigella. In Salmonella, this chromosomally encoded complex consists of over 12 genes, mutations in which abolish bacterial entry into epithelial cells. Although these genera use distinct invasion antigens, a non-invasive spa mutant of Salmonella could be rescued by the corresponding Shigella homolog. While spa promotes equivalent functions in Shigella and Salmonella, this constellation of genes has been acquired independently by each genus and displays motifs used by diverse antigen export systems including those required for flagellar assembly and protein secretion.

Related Genes
spa
MeSH Terms
Amino Acid Sequence Animals Antigens, Bacterial/genetics Antigens, Surface/genetics Base Sequence Blotting, Southern Chromosomes, Bacterial DNA Epithelial Cells Epithelium/microbiology Genes, Bacterial Genetic Complementation Test Humans Mice Molecular Sequence Data Multigene Family Mutation Restriction Mapping Salmonella typhimurium/genetics,immunology,pathogenicity Sequence Homology, Amino Acid Shigella flexneri/genetics,immunology,pathogenicity Tumor Cells, Cultured
Chemicals
Antigens, Bacterial Antigens, Surface DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Groisman E A
Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110.
Ochman H
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1993-10-00
Pages
3779-87
Language
English
Region
England
NLM ID
8208664
PMCID
PMC413660
Subset
IM
Grants
NIGMS NIH HHS · GM40995 · United States
NIAID NIH HHS · R01 AI29554 · United States
Databases
GENBANK
X73525
External Links
PubMed source PMC Full Text View journal details
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