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PMID: 1828465 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

The flaA locus of Bacillus subtilis is part of a large operon coding for flagellar structures, motility functions, and an ATPase-like polypeptide.

Journal of bacteriology ·Vol. 173 ·No. 11 ·1991-06-00 ·Pages 3573-9

Albertini AM, Caramori T, Crabb WD, Scoffone F, Galizzi A

Abstract

We cloned and sequenced 8.3 kb of Bacillus subtilis DNA corresponding to the flaA locus involved in flagellar biosynthesis, motility, and chemotaxis. The DNA sequence revealed the presence of 10 complete and 2 incomplete open reading frames. Comparison of the deduced amino acid sequences to data banks showed similarities of nine of the deduced products to a number of proteins of Escherichia coli and Salmonella typhimurium for which a role in flagellar functioning has been directly demonstrated. In particular, the sequence data suggest that the flaA operon codes for the M-ring protein, components of the motor switch, and the distal part of the basal-body rod. The gene order is remarkably similar to that described for region III of the enterobacterial flagellar regulon. One of the open reading frames was translated into a protein with 48% amino acid identity to S. typhimurium FliI and 29% identity to the beta subunit of E. coli ATP synthase.

MeSH Terms
Amino Acid Sequence Bacillus subtilis/genetics Bacterial Proteins/genetics Base Sequence Cell Movement Cloning, Molecular Flagella/physiology Flagellin Genes, Bacterial Molecular Sequence Data Open Reading Frames/genetics Operon/genetics Proton-Translocating ATPases/genetics
Chemicals
Bacterial Proteins Flagellin flaA protein, bacteria Proton-Translocating ATPases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Albertini A M
Dipartimento di Genetica e Microbiologia A. Buzzati-Traverso, Università degli Studi di Pavia, Italy.
Caramori T
Crabb W D
Scoffone F
Galizzi A
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.173.11.3573-3579.1991
Published
1991-06-00
Pages
3573-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC207974
Subset
IM
Databases
GENBANK
X56049
External Links
PubMed source DOI: 10.1128/jb.173.11.3573-3579.1991 PMC Full Text View journal details
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