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PMID: 2168368 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Developmental sensory transduction in Myxococcus xanthus involves methylation and demethylation of FrzCD.

Journal of bacteriology ·Vol. 172 ·No. 9 ·1990-09-00 ·Pages 4877-87

McCleary WR, McBride MJ, Zusman DR

Abstract

Myxococcus xanthus is a bacterium that moves by gliding motility and exhibits multicellular development (fruiting body formation). The frizzy (frz) mutants aggregate aberrantly and therefore fail to form fruiting bodies. Individual frz cells cannot control the frequency at which they reverse direction while gliding. Previously, FrzCD was shown to exhibit significant sequence similarity to the enteric methyl-accepting chemotaxis proteins. In this report, we show that FrzCD is modified by methylation and that frzF encodes the methyltransferase. We also identify a new gene, frzG, whose predicted product is homologous to that of the cheB (methylesterase) gene from Escherichia coli. Thus, although M. xanthus is unflagellated, it appears to have a sensory transduction system which is similar in many of its components to those found in flagellated bacteria.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics,isolation & purification Base Sequence Blotting, Western Chemotaxis Chromosome Mapping DNA Transposable Elements Genotype Methylation Molecular Sequence Data Mutation Myxococcales/genetics,metabolism,physiology Phenotype Sequence Homology, Nucleic Acid
Chemicals
Bacterial Proteins DNA Transposable Elements FrzCD protein, Myxococcus xanthus
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
McCleary W R
Department of Molecular and Cell Biology, University of California, Berkeley 94720.
McBride M J
Zusman D R
References (55)
55 references, click to expand
  1. Chemotaxis in Escherichia coli analysed by three-dimensional tracking.
    Nature. 1972 Oct 27;239(5374):500-4 PMID: 4563019
  2. Structure and function of bacterial sigma factors.
    Annu Rev Biochem. 1988;57:839-72 PMID: 3052291
  3. Identification of a gamma-glutamyl methyl ester in bacterial membrane protein involved in chemotaxis.
    J Biol Chem. 1977 Apr 25;252(8):2793-5 PMID: 323255
  4. Sensory transduction in Escherichia coli: two complementary pathways of information processing that involve methylated proteins.
    Proc Natl Acad Sci U S A. 1977 Aug;74(8):3312-6 PMID: 333433
  5. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  6. Isolation of bacteriophage MX4, a generalized transducing phage for Myxococcus xanthus.
    J Mol Biol. 1978 Feb 25;119(2):167-78 PMID: 416222
  7. Synergism between morphogenetic mutants of Myxococcus xanthus.
    Dev Biol. 1978 Jun;64(2):284-96 PMID: 98366
  8. A protein methylesterase involved in bacterial sensing.
    Proc Natl Acad Sci U S A. 1978 Aug;75(8):3659-63 PMID: 358191
  9. Transmembrane signaling by bacterial chemoreceptors: E. coli transducers with locked signal output.
    Cell. 1988 Dec 2;55(5):817-26 PMID: 3056621
  10. "Frizzy" aggregation genes of the gliding bacterium Myxococcus xanthus show sequence similarities to the chemotaxis genes of enteric bacteria.
    Proc Natl Acad Sci U S A. 1989 Jan;86(2):424-8 PMID: 2492105
  11. Gliding motility in Myxococcus xanthus: mgl locus, RNA, and predicted protein products.
    J Bacteriol. 1989 Feb;171(2):819-30 PMID: 2464581
  12. Three-dimensional structure of CheY, the response regulator of bacterial chemotaxis.
    Nature. 1989 Feb 23;337(6209):745-9 PMID: 2645526
  13. Genes required for developmental signalling in Myxococcus xanthus: three asg loci.
    J Bacteriol. 1989 May;171(5):2762-72 PMID: 2540156
  14. Roles of methylation and phosphorylation in the bacterial sensing system.
    Cold Spring Harb Symp Quant Biol. 1988;53 Pt 1:11-7 PMID: 3076076
  15. Phosphorylation of an N-terminal regulatory domain activates the CheB methylesterase in bacterial chemotaxis.
    J Biol Chem. 1989 Oct 15;264(29):17337-42 PMID: 2677005
  16. Evidence that the Myxococcus xanthus frz genes are developmentally regulated.
    J Bacteriol. 1989 Nov;171(11):6174-86 PMID: 2553673
  17. Role of the CheW protein in bacterial chemotaxis: overexpression is equivalent to absence.
    J Bacteriol. 1989 Nov;171(11):6271-8 PMID: 2681160
  18. Role of CheW protein in coupling membrane receptors to the intracellular signaling system of bacterial chemotaxis.
    Proc Natl Acad Sci U S A. 1989 Nov;86(22):8703-7 PMID: 2682657
  19. Protein phosphorylation and regulation of adaptive responses in bacteria.
    Microbiol Rev. 1989 Dec;53(4):450-90 PMID: 2556636
  20. Nucleotide sequence and transcriptional products of the csg locus of Myxococcus xanthus.
    J Bacteriol. 1990 Jan;172(1):15-23 PMID: 2152896
  21. Identification of the site of phosphorylation of the chemotaxis response regulator protein, CheY.
    J Biol Chem. 1989 Dec 25;264(36):21770-8 PMID: 2689446
  22. Nutritional requirements for vegetative growth of Myxococcus xanthus.
    J Bacteriol. 1962 Aug;84:250-7 PMID: 13888810
  23. Rapid visualization of protein bands in preparative SDS-polyacrylamide gels.
    Anal Biochem. 1979 Mar;93(2):257-60 PMID: 464259
  24. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  25. Multiple methylation in processing of sensory signals during bacterial chemotaxis.
    Proc Natl Acad Sci U S A. 1980 May;77(5):2429-33 PMID: 6446711
  26. Translational coupling during expression of the tryptophan operon of Escherichia coli.
    Genetics. 1980 Aug;95(4):785-95 PMID: 6162715
  27. Biochemistry of sensing and adaptation in a simple bacterial system.
    Annu Rev Biochem. 1981;50:765-82 PMID: 6791579
  28. "Frizzy" mutants: a new class of aggregation-defective developmental mutants of Myxococcus xanthus.
    J Bacteriol. 1982 Jun;150(3):1430-7 PMID: 6281244
  29. Gliding motility of Cytophaga sp. strain U67.
    J Bacteriol. 1982 Jul;151(1):384-98 PMID: 7085564
  30. Myxococcus xanthus does not respond chemotactically to moderate concentration gradients.
    J Bacteriol. 1983 Apr;154(1):437-42 PMID: 6403510
  31. Separation of signal transduction and adaptation functions of the aspartate receptor in bacterial sensing.
    Science. 1983 Jun 3;220(4601):1016-20 PMID: 6302843
  32. Interactions between chemotaxis genes and flagellar genes in Escherichia coli.
    J Bacteriol. 1983 Jul;155(1):265-74 PMID: 6305913
  33. Coliphage P1-mediated transduction of cloned DNA from Escherichia coli to Myxococcus xanthus: use for complementation and recombinational analyses.
    J Bacteriol. 1983 Jul;155(1):317-29 PMID: 6305916
  34. Surface tension gradients: feasible model for gliding motility of Myxococcus xanthus.
    J Bacteriol. 1983 Sep;155(3):1358-66 PMID: 6411689
  35. Structural similarities between the development-specific protein S from a gram-negative bacterium, Myxococcus xanthus, and calmodulin.
    Proc Natl Acad Sci U S A. 1983 Nov;80(22):6829-33 PMID: 6316328
  36. Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing.
    Gene. 1984 Jun;28(3):351-9 PMID: 6235151
  37. The relationship between base composition and codon usage in bacterial genes and its use for the simple and reliable identification of protein-coding sequences.
    Gene. 1984 Oct;30(1-3):157-66 PMID: 6096212
  38. Chimeric chemosensory transducers of Escherichia coli.
    Proc Natl Acad Sci U S A. 1985 Mar;82(5):1326-30 PMID: 3883356
  39. Cloning and complementation analysis of the "Frizzy" genes of Myxococcus xanthus.
    Mol Gen Genet. 1985;198(2):243-54 PMID: 2984519
  40. Neither methylating nor demethylating enzymes are required for bacterial chemotaxis.
    Cell. 1985 Sep;42(2):683-90 PMID: 3928170
  41. Methylation of macromolecules during development in Myxococcus xanthus.
    J Bacteriol. 1985 Nov;164(2):495-500 PMID: 3932324
  42. Nucleotide sequence corresponding to five chemotaxis genes in Escherichia coli.
    J Bacteriol. 1986 Jan;165(1):161-6 PMID: 3510184
  43. "Frizzy" genes of Myxococcus xanthus are involved in control of frequency of reversal of gliding motility.
    Proc Natl Acad Sci U S A. 1985 Dec;82(24):8767-70 PMID: 3936045
  44. Dideoxy sequencing method using denatured plasmid templates.
    Anal Biochem. 1986 Feb 1;152(2):232-8 PMID: 3516005
  45. Analysis of the products of the Myxococcus xanthus frz genes.
    J Bacteriol. 1986 May;166(2):673-8 PMID: 3009422
  46. Nucleotide sequence of the myxobacterial hemagglutinin gene contains four homologous domains.
    Proc Natl Acad Sci U S A. 1986 Sep;83(17):6332-6 PMID: 3092212
  47. Reversible staining and peptide mapping of proteins transferred to nitrocellulose after separation by sodium dodecylsulfate-polyacrylamide gel electrophoresis.
    Anal Biochem. 1986 Aug 1;156(2):341-7 PMID: 2429581
  48. Active site of the enzyme which demethylates receptors during bacterial chemotaxis.
    J Biol Chem. 1987 Jan 5;262(1):29-31 PMID: 3539934
  49. Protein phosphorylation is involved in bacterial chemotaxis.
    Proc Natl Acad Sci U S A. 1987 Nov;84(21):7609-13 PMID: 3313398
  50. Production of single-stranded plasmid DNA.
    Methods Enzymol. 1987;153:3-11 PMID: 3323803
  51. Sensory transduction in bacterial chemotaxis involves phosphotransfer between Che proteins.
    Biochem Biophys Res Commun. 1988 Mar 15;151(2):891-6 PMID: 3279958
  52. Protein phosphorylation in bacterial chemotaxis.
    Cell. 1988 Apr 8;53(1):1-2 PMID: 3280140
  53. Phosphorylation of three proteins in the signaling pathway of bacterial chemotaxis.
    Cell. 1988 Apr 8;53(1):79-87 PMID: 3280143
  54. Improved tools for biological sequence comparison.
    Proc Natl Acad Sci U S A. 1988 Apr;85(8):2444-8 PMID: 3162770
  55. Identification of a protein methyltransferase as the cheR gene product in the bacterial sensing system.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):533-7 PMID: 322131
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.172.9.4877-4887.1990
Published
1990-09-00
Pages
4877-87
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC213142
Subset
IM
Grants
NIGMS NIH HHS · GM 07232-12 · United States
NIGMS NIH HHS · GM 12356 · United States
NIGMS NIH HHS · GM 20509 · United States
Databases
GENBANK
M35200
External Links
PubMed source DOI: 10.1128/jb.172.9.4877-4887.1990 PMC Full Text View journal details
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