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PMID: 3932330 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Complementation and characterization of chemotaxis mutants of Bacillus subtilis.

Journal of bacteriology ·Vol. 164 ·No. 2 ·1985-11-00 ·Pages 802-10

Ordal GW, Parker HM, Kirby JR

Abstract

A set of chemotaxis mutants of Bacillus subtilis was complemented by using SP beta c2 transducing bacteriophage either containing cloned segments of DNA or derived from abnormal excision of SP beta c2 dl2::Tn917 inserted into the chemotaxis region. Representative mutants were characterized in capillary assays for chemotaxis toward four amino acids and mannitol and in tethered-cell experiments for addition and removal of two attractants and two repellents. Twenty complementation groups were identified, in addition to the cheR previously characterized. All were found to be defective in chemotaxis toward all chemoeffectors. They were assigned the names cheA through cheU. The large number of general chemotaxis genes in B. subtilis, in contrast to the six in Escherichia coli, suggests fundamental differences in the mechanism of chemotaxis in the two species.

MeSH Terms
Amino Acids/pharmacology Bacillus subtilis/genetics,physiology Chemotaxis Genes, Bacterial Genetic Complementation Test Mannitol/pharmacology Mutation
Chemicals
Amino Acids Mannitol
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ordal G W
Parker H M
Kirby J R
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26 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.164.2.802-810.1985
Published
1985-11-00
Pages
802-10
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC214322
Subset
IM
Grants
NIAID NIH HHS · AI20336 · United States
External Links
PubMed source DOI: 10.1128/jb.164.2.802-810.1985 PMC Full Text View journal details
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