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PMID: 3316029 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Salmonella typhimurium deletion mutants lacking adenylate cyclase and cyclic AMP receptor protein are avirulent and immunogenic.

Infection and immunity ·Vol. 55 ·No. 12 ·1987-12-00 ·Pages 3035-43

Curtiss R, Kelly SM

Abstract

Salmonella typhimurium SR-11 mutants with cya::Tn10 or crp::Tn10 mutations were found to be avirulent and immunogenic for BALB/c mice. Fusaric acid-resistant derivatives with deletions of the Tn10 and adjacent DNA sequences were constructed in S. typhimurium SR-11 strains with or without the virulence plasmid pStSR100. These delta cya delta crp strains grew more slowly than wild-type strains. They possessed wild-type ability to attach to, invade, and persist in gut-associated lymphoid tissue for up to a week but exhibited a diminished ability to reach mesenteric lymph nodes and the spleen. Mice 4 to 8 weeks old were resistant to oral infection with 10(9) cells of several different delta cya and delta cya delta crp strains (the equivalent to 10(4) 50% lethal doses of wild-type S. typhimurium SR-11) and 30 days after immunization became resistant to oral challenge with 10(3) to 10(4) 50% lethal doses of wild-type S. typhimurium SR-11.

MeSH Terms
Adenylyl Cyclases/physiology Administration, Oral Animals Immunization Lymph Nodes/microbiology Mice Mutation Peyer's Patches/microbiology Receptors, Cyclic AMP/physiology Salmonella Infections, Animal/microbiology,prevention & control Salmonella typhimurium/genetics,immunology,pathogenicity Tissue Distribution
Chemicals
Receptors, Cyclic AMP Adenylyl Cyclases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Curtiss R
Department of Biology, Washington University, St. Louis, Missouri 63130.
Kelly S M
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.55.12.3035-3043.1987
Published
1987-12-00
Pages
3035-43
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC260025
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.55.12.3035-3043.1987 PMC Full Text View journal details
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