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PMID: 3316027 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Plasmid-associated virulence of Salmonella typhimurium.

Infection and immunity ·Vol. 55 ·No. 12 ·1987-12-00 ·Pages 2891-901

Gulig PA, Curtiss R

Abstract

We investigated the role of the 100-kilobase (kb) plasmid of Salmonella typhimurium in the virulence of this organism for mice. Three strains, LT2-Z, SR-11, and SL1344, which possessed 100-kb plasmids with identical restriction enzyme digestion profiles, were cured of their respective 100-kb plasmids after Tnmini-tet was used to label plasmids. Curing wild-type virulent strains SR-11 and SL1344 raised peroral 50% lethal doses from 3 x 10(5) and 6 x 10(4) CFU, respectively, to greater than 10(8) CFU. Both wild-type strains had intraperitoneal 50% lethal doses of less than 50 CFU, whereas the intraperitoneal 50% lethal doses for cured SR-11 and SL1344 were less than 50 and 400 CFU, respectively. Reintroduction of the Tnmini-tet-labeled, 100-kb plasmid restored wild-type virulence. Invasion from Peyer's patches to mesenteric lymph nodes and spleens after peroral inoculation was the stage of pathogenesis most affected by curing S. typhimurium of the 100-kb plasmid. Wild-type S. typhimurium replicated in spleens of mice inoculated intravenously to a greater extent than did plasmid-cured derivatives. Wild-type and cured strains equally adhered to and invaded Henle-407, HEp-2, and CHO cells; furthermore, the presence of the 100-kb plasmid was not necessary for replication of S. typhimurium within CHO cells. The 100-kb plasmid had no effect on phagocytosis and killing of S. typhimurium by murine peritoneal macrophages in vitro and in vivo. Similarly, wild-type and plasmid-cured strains were resistant to killing by 90% normal human, rabbit, and guinea pig sera. All wild-type and plasmid-cured S. typhimurium strains possessed complete lipopolysaccharide, as determined by silver staining solubilized cells in sodium dodecyl sulfate-polyacrylamide gels. We have confirmed the role of the 100-kb plasmid of S. typhimurium in virulence, primarily in invasion to mesenteric lymph nodes and spleens after peroral inoculation of mice. Involvement of the 100-kb plasmid in infection of mesenteric lymph nodes and spleens suggests a role for the plasmid in the complex interaction of S. typhimurium with cells of the reticuloendothelial system.

MeSH Terms
Animals Bacterial Adhesion Blood Bactericidal Activity Cell Line Congo Red/metabolism Culture Media DNA, Bacterial/genetics Macrophages/microbiology Mice Peyer's Patches/microbiology Plasmids Salmonella Infections, Animal/microbiology Salmonella typhimurium/genetics,pathogenicity Spleen/microbiology
Chemicals
Culture Media DNA, Bacterial Congo Red
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gulig P A
Department of Biology, Washington University, St. Louis, Missouri 63130.
Curtiss R
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.55.12.2891-2901.1987
Published
1987-12-00
Pages
2891-901
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC260003
Subset
IM
Grants
NIAID NIH HHS · F32-AI07168 · United States
NIDCR NIH HHS · R01-DE06669 · United States
External Links
PubMed source DOI: 10.1128/iai.55.12.2891-2901.1987 PMC Full Text View journal details
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