Home LiteratureArticle Details
PMID: 3312005 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Plasmid-associated resistance of Salmonella typhimurium to complement activated by the classical pathway.

Infection and immunity ·Vol. 55 ·No. 11 ·1987-11-00 ·Pages 2645-52

Vandenbosch JL, Rabert DK, Jones GW

Abstract

The association of the virulence plasmid of Salmonella typhimurium with resistance to the bactericidal activity of human serum was studied in chromosomally isogenic pairs of strains differing in their virulence plasmid status. The presence of the plasmid correlated in three pairs of strains with resistance to serum. The absence of the plasmid correlated with increased sensitivity to serum, whereas the reintroduction of the plasmid to the cell resulted in the restoration of resistance to serum. Complement was activated by the classical and alternative pathways equally well by both strains of a pair, but the differential bactericidal action of serum was apparent only after the activation of complement by the classical pathway. No differences in the chemical compositions or in the molecular weight ranges of the lipopolysaccharides were apparent between paired strains. This work confirms the presence of a virulence plasmid-associated mechanism of resistance to serum and distinguishes it from lipopolysaccharide-mediated resistance.

MeSH Terms
Blood Bactericidal Activity Complement Activation Complement Pathway, Classical Lipopolysaccharides/immunology Molecular Weight Mutation Plasmids Salmonella Phages/growth & development Salmonella typhimurium/genetics,immunology,pathogenicity
Chemicals
Lipopolysaccharides
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Vandenbosch J L
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor 48109.
Rabert D K
Jones G W
References (46)
46 references, click to expand
  1. Antibacterial peptide from normal rat serum. 1. Isolation from whole serum, activity, and microbicidal spectrum.
    Biochemistry. 1981 Oct 13;20(21):5973-81 PMID: 6796108
  2. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  3. Activation of complement by opportunist pathogens and chemotypes of Salmonella minnesota.
    Infect Immun. 1977 Jun;16(3):748-53 PMID: 408268
  4. traT gene sequences, serum resistance and pathogenicity-related factors in clinical isolates of Escherichia coli and other gram-negative bacteria.
    J Gen Microbiol. 1985 Jun;131(6):1511-21 PMID: 3900279
  5. Transduction by bacteriophage P22 in nonsmooth mutants of Salmonella typhimurium.
    J Bacteriol. 1967 May;93(5):1588-97 PMID: 5337845
  6. Complement activation by polysaccharide of lipopolysaccharide: an important virulence determinant of salmonellae.
    Infect Immun. 1983 Aug;41(2):563-9 PMID: 6347890
  7. Differential complement activation and susceptibility to human serum bactericidal action by Vibrio species.
    Infect Immun. 1983 Dec;42(3):1187-90 PMID: 6642665
  8. Functional relationship of factor B in the properdin system to C3 proactivator of human serum.
    J Immunol. 1971 Oct;107(4):1200-4 PMID: 5093780
  9. Antibody-independent binding of the first component of complement (C1) and its subcomponent C1q to the S and R forms of Salmonella minnesota.
    Infect Immun. 1981 Mar;31(3):1138-44 PMID: 6971812
  10. Plasmid-determined alterations of Salmonella typhimurium lipopolysaccharides.
    Mol Gen Genet. 1975 Sep 29;140(2):175-81 PMID: 1105157
  11. Serum-resistant mutants of Escherichia coli O111 contain increased lipopolysaccharide, lack an O antigen-containing capsule, and cover more of their lipid A core with O antigen.
    J Bacteriol. 1984 Sep;159(3):877-82 PMID: 6207166
  12. Environmental modulation of lipopolysaccharide chain length alters the sensitivity of Escherichia coli to the neutrophil bactericidal/permeability-increasing protein.
    Infect Immun. 1986 Feb;51(2):594-9 PMID: 3510983
  13. Activation of the alternate pathway of human complements by rabbit cells.
    J Immunol. 1974 Jul;113(1):348-58 PMID: 4134064
  14. Bactericidal and bacteriolytic activity of serum against gram-negative bacteria.
    Microbiol Rev. 1983 Mar;47(1):46-83 PMID: 6343827
  15. Antibacterial peptide from normal rabbit serum. 2. Compositional microanalysis.
    Biochemistry. 1981 Oct 13;20(21):5981-7 PMID: 7306487
  16. Endotoxic glycolipid from a heptoseless mutant of Salmonella minnesota.
    J Bacteriol. 1967 Dec;94(6):1824-36 PMID: 4965363
  17. High-molecular-weight components in lipopolysaccharides of Salmonella typhimurium, Salmonella minnesota, and Escherichia coli.
    J Bacteriol. 1985 May;162(2):738-45 PMID: 3886631
  18. Rapid procedure for detection and isolation of large and small plasmids.
    J Bacteriol. 1981 Mar;145(3):1365-73 PMID: 7009583
  19. Activation of complement system by porins extracted from Salmonella typhimurium.
    Infect Immun. 1984 Nov;46(2):559-63 PMID: 6094352
  20. Salmonellae activate complement differentially via the alternative pathway depending on the structure of their lipopolysaccharide O-antigen.
    J Immunol. 1983 Apr;130(4):1867-70 PMID: 6187823
  21. Studies on the mechanism of bacterial resistance to complement-mediated killing. II. C8 and C9 release C5b67 from the surface of Salmonella minnesota S218 because the terminal complex does not insert into the bacterial outer membrane.
    J Exp Med. 1982 Mar 1;155(3):809-19 PMID: 6801180
  22. Heterogeneity of antigenic-side-chain length in lipopolysaccharide from Escherichia coli 0111 and Salmonella typhimurium LT2.
    Eur J Biochem. 1980;107(1):145-53 PMID: 6156828
  23. Decreased binding of polymyxin by polymyxin-resistant mutants of Salmonella typhimurium.
    J Bacteriol. 1979 Aug;139(2):664-7 PMID: 222738
  24. Opsonophagocytosis of Neisseria gonorrhoeae: interaction of local and disseminated isolates with complement and neutrophils.
    J Infect Dis. 1985 Jan;151(1):33-41 PMID: 3917484
  25. Lipid A and resistance of Salmonella typhimurium to antimicrobial granule proteins of human neutrophil granulocytes.
    Infect Immun. 1984 Mar;43(3):834-8 PMID: 6199303
  26. Lipopolysaccharide heterogeneity in Salmonella typhimurium analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis.
    Eur J Biochem. 1980;107(1):137-43 PMID: 6995111
  27. The heterocytotoxicity of human serum. I. Activation of the alternative complement pathway by heterologous target cells.
    Cell Immunol. 1977 Mar 1;29(1):174-86 PMID: 403014
  28. Mapping of genes determining penicillin-resistance and serum-sensitivity in Salmonella enteritidis.
    J Gen Microbiol. 1980 Jun;118(2):367-76 PMID: 7003058
  29. Studies on the mechanism of bacterial resistance to complement-mediated killing. I. Terminal complement components are deposited and released from Salmonella minnesota S218 without causing bacterial death.
    J Exp Med. 1982 Mar 1;155(3):797-808 PMID: 6801179
  30. Analyses of lipopolysaccharides extracted from penicillin-resistant, serum-sensitive salmonella mutants.
    J Gen Microbiol. 1967 Aug;48(2):179-88 PMID: 5341578
  31. Analysis of outer membrane components of Escherichia coli ML308 225 and of a serum-resistant mutant.
    Infect Immun. 1980 May;28(2):387-92 PMID: 6995308
  32. Killing of the S and Re forms of Salmonella minnesota via the classical pathway of complement activation in guinea-pig and human sera.
    Immunology. 1980 Aug;40(4):547-56 PMID: 6776034
  33. Inhibition of serum bactericidal reaction by lipopolysaccharide.
    Infect Immun. 1985 Jun;48(3):759-62 PMID: 4039705
  34. Responses of marine bacteria under starvation conditions at a solid-water interface.
    Appl Environ Microbiol. 1983 Jan;45(1):43-7 PMID: 16346180
  35. The sensitivity of smooth and rough mutants of Salmonella typhimurium to bactericidal and bacteriolytic action of serum, lysozyme and to phagocytosis.
    Folia Microbiol (Praha). 1968;13(5):439-49 PMID: 4880678
  36. Mediation of serum resistance in Salmonella typhimurium by an 11-kilodalton polypeptide encoded by the cryptic plasmid.
    J Infect Dis. 1987 Mar;155(3):540-9 PMID: 3543157
  37. Epidemiology of virulence-associated plasmids and outer membrane protein patterns within seven common Salmonella serotypes.
    Infect Immun. 1985 Apr;48(1):175-82 PMID: 3980081
  38. Physical, chemical, and immunological properties of lipopolysaccharide released from Escherichia coli by ethylenediaminetetraacetate.
    J Biol Chem. 1968 Dec 25;243(24):6384-91 PMID: 4973230
  39. Neisseria gonorrhoeae acquire a new principal outer-membrane protein when transformed to resistance to serum bactericidal activity.
    Infect Immun. 1978 Apr;20(1):267-72 PMID: 78895
  40. Role of surface components in serum resistance of virulent Aeromonas salmonicida.
    Infect Immun. 1982 Jun;36(3):1069-75 PMID: 7095843
  41. Interrelationship between serum beta-lysin, lysozyme, and the antibody-complement system in killing Escherichia coli.
    Infect Immun. 1974 Sep;10(3):657-66 PMID: 4609906
  42. A study of optimal reaction conditions for an assay of the human alternative complement pathway.
    Am J Clin Pathol. 1983 Jan;79(1):65-72 PMID: 6849295
  43. Non-smooth mutants of Salmonella typhimurium: differentiation by phage sensitivity and genetic mapping.
    J Gen Microbiol. 1972 May;70(3):527-54 PMID: 4556257
  44. Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels.
    J Bacteriol. 1983 Apr;154(1):269-77 PMID: 6187729
  45. A protein factor associated with serum resistance in Escherichia coli.
    J Med Microbiol. 1977 May;10(2):225-32 PMID: 323489
  46. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels.
    Anal Biochem. 1982 Jan 1;119(1):115-9 PMID: 6176137
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.55.11.2645-2652.1987
Published
1987-11-00
Pages
2645-52
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC259955
Subset
IM
Grants
NIAID NIH HHS · AI19647 · United States
External Links
PubMed source DOI: 10.1128/iai.55.11.2645-2652.1987 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2