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PMID: 2544889 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A two-component regulatory system (phoP phoQ) controls Salmonella typhimurium virulence.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 13 ·1989-07-00 ·Pages 5054-8

Miller SI, Kukral AM, Mekalanos JJ

Abstract

We have determined that Salmonella typhimurium strains with mutations in the positive regulatory locus phoP are markedly attenuated in virulence for BALB/c mice. The DNA sequence for the phoP locus indicates that it is composed of two genes present in an operon, termed phoP and phoQ. The deduced amino acid sequence of the phoP and phoQ gene products are highly similar to other members of bacterial two-component transcriptional regulators that respond to environmental stimuli. S. typhimurium strains with transposon insertions that create transcriptional and translational gene fusions that require phoP and phoQ for expression have been isolated and have different chromosomal locations, indicating that this system is a regulon. One of these fusion strains, containing a mutation in a gene termed pagC, has a virulence defect. Other strains, including those containing mutations in the phoN gene, encoding an acid phosphatase, have wild-type virulence. Strains with pagC, phoP, or phoQ mutations have decreased survival in cultured mouse macrophages. When used as live vaccines in mice, strains with phoP or phoQ mutations afford partial protection to subsequent challenge by wild-type S. typhimurium.

MeSH Terms
Amino Acid Sequence Animals Base Sequence DNA Transposable Elements Genes, Bacterial Genes, Regulator Genotype Mice Molecular Sequence Data Mutation Salmonella Infections/microbiology Salmonella typhimurium/genetics,pathogenicity Virulence
Chemicals
DNA Transposable Elements
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Miller S I
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115.
Kukral A M
Mekalanos J J
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.13.5054
Published
1989-07-00
Pages
5054-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC297555
Subset
IM
Grants
NIAID NIH HHS · AI-18045 · United States
NIAID NIH HHS · AI-26289 · United States
Databases
GENBANK
M24424
External Links
PubMed source DOI: 10.1073/pnas.86.13.5054 PMC Full Text View journal details
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