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PMID: 2999794 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

TnphoA: a transposon probe for protein export signals.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 23 ·1985-12-00 ·Pages 8129-33

Manoil C, Beckwith J

Abstract

We constructed a derivative of transposon Tn5 that permits the generation of hybrid proteins composed of alkaline phosphatase (EC 3.1.3.1) lacking its signal peptide fused to amino-terminal sequences of other proteins. Such a hybrid gives alkaline phosphatase activity if the protein fused to alkaline phosphatase contributes sequences that promote export and thus compensate for the missing alkaline phosphatase signal peptide. Fusions to both a secreted periplasmic protein and a complex cytoplasmic membrane protein led to alkaline phosphatase activity. TnphoA fusions should help localize export signals within the structure of a protein, such as a transmembrane protein, as well as identify new chromosomal genes for secreted and transmembrane proteins.

MeSH Terms
Alkaline Phosphatase/genetics Base Sequence Biological Transport Cell Compartmentation Codon DNA Transposable Elements DNA, Bacterial/genetics DNA, Recombinant Escherichia coli/genetics Genetic Vectors Protein Sorting Signals/genetics beta-Galactosidase/genetics
Chemicals
Codon DNA Transposable Elements DNA, Bacterial DNA, Recombinant Protein Sorting Signals Alkaline Phosphatase beta-Galactosidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Manoil C
Beckwith J
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28 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.23.8129
Published
1985-12-00
Pages
8129-33
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC391456
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.82.23.8129 PMC Full Text View journal details
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