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PMID: 1332940 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

MxiJ, a lipoprotein involved in secretion of Shigella Ipa invasins, is homologous to YscJ, a secretion factor of the Yersinia Yop proteins.

Journal of bacteriology ·Vol. 174 ·No. 23 ·1992-12-00 ·Pages 7661-9

Allaoui A, Sansonetti PJ, Parsot C

Abstract

Shigella flexneri causes bacillary dysentery by invading epithelial cells of the colonic mucosa. The invasion process requires the synthesis and secretion of the virulence plasmid-encoded Ipa proteins. Using TnphoA mutagenesis, we have identified two virulence plasmid genes, mxiJ and mxiM, that encode proteins exported by the general export pathway. Analysis of the MxiJ and MxiM deduced amino acid sequences suggested that mxiJ and mxiM might encode lipoproteins, which was confirmed by [3H]palmitate labeling of MxiJ:PhoA and MxiM:PhoA fusion proteins. A mxiJ mutant was unable to invade HeLa cells, to induce the formation of plaques on confluent monolayers of HeLa cells, and to provoke keratoconjunctivitis in guinea pigs. In addition, secretion of seven polypeptides, including IpaA, IpaB, and IpaC, was abolished in the mxiJ mutant. Sequence comparisons indicated that MxiJ and MxiH, which is encoded by a gene located upstream from mxiJ, are homologous to the Yersinia enterocolitica YscJ and YscF proteins, respectively.

MeSH Terms
Adhesins, Bacterial Animals Bacterial Outer Membrane Proteins Bacterial Proteins/genetics Base Sequence DNA Transposable Elements Escherichia coli/genetics Genes, Bacterial/genetics Guinea Pigs HeLa Cells Humans Lipoproteins/genetics,metabolism Molecular Sequence Data Mutagenesis, Insertional Palmitic Acid Palmitic Acids/metabolism Plasmids/genetics Sequence Homology, Amino Acid Shigella flexneri/genetics,pathogenicity Virulence
Chemicals
Adhesins, Bacterial Bacterial Outer Membrane Proteins Bacterial Proteins DNA Transposable Elements Lipoproteins MxiH protein, Shigella Palmitic Acids invasin, Yersinia YscJ protein, Yersinia enterocolitica mxiJ protein, Shigella flexneri mxiM protein, Shigella flexneri Palmitic Acid
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Allaoui A
Unité de Pathogénie Microbienne Moléculaire, Unité INSERM 199, Institut Pasteur, Paris, France.
Sansonetti P J
Parsot C
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.174.23.7661-7669.1992
Published
1992-12-00
Pages
7661-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC207479
Subset
IM
Databases
GENBANK
L01135, L01136, L01137, L01138, L01139, L01140, L01141, M98390, M98391, Z11768
External Links
PubMed source DOI: 10.1128/jb.174.23.7661-7669.1992 PMC Full Text View journal details
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