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PMID: 1582426 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

IpaB of Shigella flexneri causes entry into epithelial cells and escape from the phagocytic vacuole.

The EMBO journal ·Vol. 11 ·No. 5 ·1992-05-00 ·Pages 1991-9

High N, Mounier J, Prévost MC, Sansonetti PJ

Abstract

By creating mutations within the Shigella flexneri ipaB gene, we have demonstrated that the invasion of epithelial cells is a three-step process encompassing adhesion on the cell surface, entry and lysis of the phagocytic vacuole allowing subsequent access to the cytoplasm. SC403, an insertion mutant which lacks expression of IpaB but still expresses downstream genes, has been particularly studied. It is non-invasive, does not elicit actin polymerization, but binds to HeLa cells indicating that an adhesion step occurs immediately prior to the entry process. The consequence of the inactivation of ipaB on the intracellular behaviour of S.flexneri was investigated using the macrophage cell line J774. SC403 was unable to lyse the phagocytic vacuole; moreover, this strain did not display the contact mediated haemolytic activity characteristics of Shigella. In addition to being a major component of the invasion complex, IpaB acts as a membrane-lysing toxin enabling escape to the cytoplasmic compartment.

Related Genes
ipaB
MeSH Terms
Bacterial Adhesion Blotting, Western Epithelial Cells Epithelium/microbiology Gene Expression Genes, Bacterial HeLa Cells Humans Macrophages/microbiology Mutation Phagocytes/microbiology Plasmids Restriction Mapping Shigella flexneri/genetics,pathogenicity,physiology Vacuoles/microbiology Virulence/genetics
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
High N
Unité de Pathogénie Microbienne Moléculaire, Unité INSERM 199, Institut Pasteur, Paris, France.
Mounier J
Prévost M C
Sansonetti P J
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1992-05-00
Pages
1991-9
Language
English
Region
England
NLM ID
8208664
PMCID
PMC556659
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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