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PMID: 2833557 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Role of hemolysin for the intracellular growth of Listeria monocytogenes.

The Journal of experimental medicine ·Vol. 167 ·No. 4 ·1988-04-01 ·Pages 1459-71

Portnoy DA, Jacks PS, Hinrichs DJ

Abstract

Listeria monocytogenes insertion mutants defective in hemolysin production were generated using the conjugative transposons Tn916 and Tn1545. All of the nonhemolytic mutants (hly-) lacked a secreted 58-kD polypeptide, presumedly hemolysin, and were avirulent in a mouse model. An intracellular multiplication assay was established in monolayers of mouse bone marrow-derived macrophages, the J774 macrophage-like cell line, the CL.7 embryonic mouse fibroblast cell line, and the Henle 407 human epithelial cell line. The hly+ strain grew intracellularly in all of the tissue culture cells with a doubling time of approximately 60 min. In contrast, the hly- mutants failed to grow in the murine-derived tissue culture cells, but retained the ability to grow in the human tissue culture cells examined. Hemolytic-positive revertants were selected after passage of the hly- mutants through monolayers of J774 cells. In each case, the hemolytic revertants possessed the 58-kD polypeptide, were capable of intracellular growth in tissue culture monolayers and were virulent for mice.

MeSH Terms
Animals Bacterial Proteins/genetics,physiology Cell Line DNA Transposable Elements Epithelial Cells Epithelium/microbiology Fibroblasts/microbiology Hemolysin Proteins/genetics,physiology Humans Listeria monocytogenes/cytology,genetics Macrophages/microbiology Mice Organ Specificity Species Specificity
Chemicals
Bacterial Proteins DNA Transposable Elements Hemolysin Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Portnoy D A
Department of Microbiology and Immunology, School of Medicine, Washington University, St. Louis, Missouri 63110.
Jacks P S
Hinrichs D J
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.167.4.1459
Published
1988-04-01
Pages
1459-71
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2188911
Subset
IM
Grants
NCRR NIH HHS · 2-SO7-RR05389 · United States
NIAID NIH HHS · 7-F32-AI-06918 · United States
NIAID NIH HHS · AI-23455 · United States
External Links
PubMed source DOI: 10.1084/jem.167.4.1459 PMC Full Text View journal details
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