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PMID: 2000374 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

ToxR regulates the production of lipoproteins and the expression of serum resistance in Vibrio cholerae.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 5 ·1991-03-01 ·Pages 1641-5

Parsot C, Taxman E, Mekalanos JJ

Abstract

The genes encoding three lipoproteins of Vibrio cholerae were identified by a combination of DNA sequence analysis and [3H]palmitate labeling of hybrid proteins encoded by TnphoA gene fusions. The expression of these three lipoproteins, TagA, AcfD, and TcpC, was controlled by ToxR, the cholera toxin transcriptional activator. The involvement of other bacterial lipoproteins in conferring resistance to the bactericidal effects of complement prompted us to examine this possibility in V. cholerae. Remarkably, mutations in toxR and tcp genes (including tcpC), involved in the biogenesis of the toxin coregulated pili, rendered V. cholerae about 10(4)-10(6) times more sensitive to the vibriocidal activity of antibody and complement. Since V. cholerae is a noninvasive organism and toxR and tcp mutants are highly defective in intestinal colonization in animals and humans, these results raise the possibility that resistance to a gut-associated, "complement-like" bactericidal activity may be a major virulence determinant of V. cholerae and other enterobacterial species.

MeSH Terms
Amino Acid Sequence Animals Antibodies, Bacterial/immunology Bacterial Outer Membrane Proteins/genetics,isolation & purification Bacterial Proteins Complement System Proteins/immunology DNA-Binding Proteins/genetics,metabolism Genotype Guinea Pigs Lipoproteins/genetics,immunology Molecular Sequence Data Palmitic Acid Palmitic Acids/metabolism Recombinant Fusion Proteins/analysis Transcription Factors/genetics,metabolism Vibrio cholerae/genetics,immunology,pathogenicity Virulence/genetics
Chemicals
Antibodies, Bacterial Bacterial Outer Membrane Proteins Bacterial Proteins DNA-Binding Proteins Lipoproteins Palmitic Acids Recombinant Fusion Proteins Transcription Factors toxR protein, Vibrio cholerae toxR protein, bacteria Palmitic Acid Complement System Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Parsot C
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115.
Taxman E
Mekalanos J J
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.5.1641
Published
1991-03-01
Pages
1641-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC51080
Subset
IM
Grants
NIAID NIH HHS · AI-18405 · United States
NIAID NIH HHS · AI-26289 · United States
External Links
PubMed source DOI: 10.1073/pnas.88.5.1641 PMC Full Text View journal details
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