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PMID: 2883655 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Use of phoA gene fusions to identify a pilus colonization factor coordinately regulated with cholera toxin.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 9 ·1987-05-00 ·Pages 2833-7

Taylor RK, Miller VL, Furlong DB, Mekalanos JJ

Abstract

The transposon TnphoA was used to generate fusions between phoA, the gene for alkaline phosphatase (PhoA), and genes encoding proteins that are secreted by Vibrio cholerae. One of the PhoA+ mutants isolated showed a dramatic reduction in its ability to colonize the intestines of suckling mice. This mutant no longer produced a 20.5-kDa protein (TcpA) that we show is the major subunit of a V. cholerae pilus. Amino-terminal sequence analysis of the TcpA pilus subunit showed that it shares amino acid homology with the pilins produced by several other pathogenic bacteria. The TcpA pilus was coordinately expressed with cholera toxin under various culture conditions, and this effect appeared to be dependent on the transcriptional activator encoded by the toxR gene. We conclude that the toxR gene plays a central role in the transcriptional regulation of multiple virulence genes of V. cholerae.

MeSH Terms
Alkaline Phosphatase/genetics Amino Acid Sequence Cholera Toxin/genetics Fimbriae, Bacterial/physiology,ultrastructure Gene Expression Regulation Genes Genes, Bacterial Microscopy, Electron Mutation Species Specificity Vibrio cholerae/enzymology,genetics,ultrastructure
Chemicals
Cholera Toxin Alkaline Phosphatase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Taylor R K
Miller V L
Furlong D B
Mekalanos J J
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21 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.9.2833
Published
1987-05-00
Pages
2833-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC304754
Subset
IM
Grants
NIAID NIH HHS · 5 F32 AI06993-02 BI-1 · United States
NIGMS NIH HHS · 5T32 GM07196 · United States
NIAID NIH HHS · AI18045 · United States
External Links
PubMed source DOI: 10.1073/pnas.84.9.2833 PMC Full Text View journal details
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